Sequence and genomic organization of the mouse Lim1 gene

Yuanhao Li; Siew Sim Cheah; Jian Min Deng; William Shawlot; Richard R. Behringer

doi:10.1007/s003359901020

Sequence and genomic organization of the mouse Lim1 gene

Yuanhao Li, Siew Sim Cheah, Jian Min Deng, William Shawlot, Richard R. Behringer

Genetics

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The sequence and genomic organization of the mouse Lim1 gene were determined. The mouse Lim1 gene has five coding exons. The Lim1 transcription initiation start site was determined by 5' RACE, indicating that the first exon encodes the translation initiation codon and a 1360-bp 5' untranslated region. Sequence analysis of the 450-bp upstream of the transcription start site revealed the presence of a CATTAA motif at -32 bp and a CAATT box located in reverse orientation at -68 bp. HNF3β and Pbx1 binding sites were also identified. Like most LIM domain encoding genes, the LIM domains of Lim1 are each encoded on separate and adjacent exons. Knowledge of the sequence and structure of the mouse Lim1 gene provides important information for the genetic manipulation of the Lim1 locus.

Original language	English (US)
Pages (from-to)	444-446
Number of pages	3
Journal	Mammalian Genome
Volume	10
Issue number	5
DOIs	https://doi.org/10.1007/s003359901020
State	Published - 1999

ASJC Scopus subject areas

Genetics

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10.1007/s003359901020

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title = "Sequence and genomic organization of the mouse Lim1 gene",

abstract = "The sequence and genomic organization of the mouse Lim1 gene were determined. The mouse Lim1 gene has five coding exons. The Lim1 transcription initiation start site was determined by 5' RACE, indicating that the first exon encodes the translation initiation codon and a 1360-bp 5' untranslated region. Sequence analysis of the 450-bp upstream of the transcription start site revealed the presence of a CATTAA motif at -32 bp and a CAATT box located in reverse orientation at -68 bp. HNF3β and Pbx1 binding sites were also identified. Like most LIM domain encoding genes, the LIM domains of Lim1 are each encoded on separate and adjacent exons. Knowledge of the sequence and structure of the mouse Lim1 gene provides important information for the genetic manipulation of the Lim1 locus.",

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year = "1999",

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T1 - Sequence and genomic organization of the mouse Lim1 gene

AU - Li, Yuanhao

AU - Cheah, Siew Sim

AU - Deng, Jian Min

AU - Shawlot, William

AU - Behringer, Richard R.

PY - 1999

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N2 - The sequence and genomic organization of the mouse Lim1 gene were determined. The mouse Lim1 gene has five coding exons. The Lim1 transcription initiation start site was determined by 5' RACE, indicating that the first exon encodes the translation initiation codon and a 1360-bp 5' untranslated region. Sequence analysis of the 450-bp upstream of the transcription start site revealed the presence of a CATTAA motif at -32 bp and a CAATT box located in reverse orientation at -68 bp. HNF3β and Pbx1 binding sites were also identified. Like most LIM domain encoding genes, the LIM domains of Lim1 are each encoded on separate and adjacent exons. Knowledge of the sequence and structure of the mouse Lim1 gene provides important information for the genetic manipulation of the Lim1 locus.

AB - The sequence and genomic organization of the mouse Lim1 gene were determined. The mouse Lim1 gene has five coding exons. The Lim1 transcription initiation start site was determined by 5' RACE, indicating that the first exon encodes the translation initiation codon and a 1360-bp 5' untranslated region. Sequence analysis of the 450-bp upstream of the transcription start site revealed the presence of a CATTAA motif at -32 bp and a CAATT box located in reverse orientation at -68 bp. HNF3β and Pbx1 binding sites were also identified. Like most LIM domain encoding genes, the LIM domains of Lim1 are each encoded on separate and adjacent exons. Knowledge of the sequence and structure of the mouse Lim1 gene provides important information for the genetic manipulation of the Lim1 locus.

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Sequence and genomic organization of the mouse Lim1 gene

Abstract

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