Serine-derivatized gadonanotubes as magnetic nanoprobes for intracellular labeling

Amy A. Hassan; Bonita Tak Yee Chan; Lesa A. Tran; Keith B. Hartman; Jeyarama S. Ananta; Yuri Mackeyev; Lingyun Hu; Robia G. Pautler; Lon J. Wilson; Adrian V. Lee

doi:10.1002/cmmi.293

Serine-derivatized gadonanotubes as magnetic nanoprobes for intracellular labeling

Amy A. Hassan, Bonita Tak Yee Chan, Lesa A. Tran, Keith B. Hartman, Jeyarama S. Ananta, Yuri Mackeyev, Lingyun Hu, Robia G. Pautler, Lon J. Wilson, Adrian V. Lee

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Gadonanotubes (GNTs), which are powerful new T₁-weighted MRI contrast agents, were derivatized with serine amino acid substituents to produce water-soluble (2 mg ml^-1) ser-gadonanotubes (ser-GNs) as magnetic nanoprobes for intracellular labeling. The ser-GNTs were used to efficiently label MCF-7 human breast cancer cells (1.5 × 10⁹ Gd ³⁺ ions/cell) with no observable cytotoxicity. Cell pellets derived from the ser-GNT labeled cells give bright T₁-weighted MR images, confirming that the ser-GNTs are a promising new nanoprobe technology for magnetic cell labeling and possibly for in vivo cellular trafficking.

Original language	English (US)
Pages (from-to)	34-38
Number of pages	5
Journal	Contrast Media and Molecular Imaging
Volume	5
Issue number	1
DOIs	https://doi.org/10.1002/cmmi.293
State	Published - Jan 2010
Externally published	Yes

Keywords

Breast cancer
Carbon nanotubes
Gadonanotubes
Magnetic resonance imaging (MRI)
Nanotechnology

ASJC Scopus subject areas

Radiology Nuclear Medicine and imaging

Access to Document

10.1002/cmmi.293

Cite this

@article{4eb3a0d68a7e4e5b8018e25058458d9b,

title = "Serine-derivatized gadonanotubes as magnetic nanoprobes for intracellular labeling",

abstract = "Gadonanotubes (GNTs), which are powerful new T1-weighted MRI contrast agents, were derivatized with serine amino acid substituents to produce water-soluble (2 mg ml-1) ser-gadonanotubes (ser-GNs) as magnetic nanoprobes for intracellular labeling. The ser-GNTs were used to efficiently label MCF-7 human breast cancer cells (1.5 × 109 Gd 3+ ions/cell) with no observable cytotoxicity. Cell pellets derived from the ser-GNT labeled cells give bright T1-weighted MR images, confirming that the ser-GNTs are a promising new nanoprobe technology for magnetic cell labeling and possibly for in vivo cellular trafficking.",

keywords = "Breast cancer, Carbon nanotubes, Gadonanotubes, Magnetic resonance imaging (MRI), Nanotechnology",

author = "Hassan, {Amy A.} and Chan, {Bonita Tak Yee} and Tran, {Lesa A.} and Hartman, {Keith B.} and Ananta, {Jeyarama S.} and Yuri Mackeyev and Lingyun Hu and Pautler, {Robia G.} and Wilson, {Lon J.} and Lee, {Adrian V.}",

year = "2010",

month = jan,

doi = "10.1002/cmmi.293",

language = "English (US)",

volume = "5",

pages = "34--38",

journal = "Contrast Media and Molecular Imaging",

issn = "1555-4309",

publisher = "John Wiley and Sons Ltd",

number = "1",

}

TY - JOUR

T1 - Serine-derivatized gadonanotubes as magnetic nanoprobes for intracellular labeling

AU - Hassan, Amy A.

AU - Chan, Bonita Tak Yee

AU - Tran, Lesa A.

AU - Hartman, Keith B.

AU - Ananta, Jeyarama S.

AU - Mackeyev, Yuri

AU - Hu, Lingyun

AU - Pautler, Robia G.

AU - Wilson, Lon J.

AU - Lee, Adrian V.

PY - 2010/1

Y1 - 2010/1

N2 - Gadonanotubes (GNTs), which are powerful new T1-weighted MRI contrast agents, were derivatized with serine amino acid substituents to produce water-soluble (2 mg ml-1) ser-gadonanotubes (ser-GNs) as magnetic nanoprobes for intracellular labeling. The ser-GNTs were used to efficiently label MCF-7 human breast cancer cells (1.5 × 109 Gd 3+ ions/cell) with no observable cytotoxicity. Cell pellets derived from the ser-GNT labeled cells give bright T1-weighted MR images, confirming that the ser-GNTs are a promising new nanoprobe technology for magnetic cell labeling and possibly for in vivo cellular trafficking.

AB - Gadonanotubes (GNTs), which are powerful new T1-weighted MRI contrast agents, were derivatized with serine amino acid substituents to produce water-soluble (2 mg ml-1) ser-gadonanotubes (ser-GNs) as magnetic nanoprobes for intracellular labeling. The ser-GNTs were used to efficiently label MCF-7 human breast cancer cells (1.5 × 109 Gd 3+ ions/cell) with no observable cytotoxicity. Cell pellets derived from the ser-GNT labeled cells give bright T1-weighted MR images, confirming that the ser-GNTs are a promising new nanoprobe technology for magnetic cell labeling and possibly for in vivo cellular trafficking.

KW - Breast cancer

KW - Carbon nanotubes

KW - Gadonanotubes

KW - Magnetic resonance imaging (MRI)

KW - Nanotechnology

UR - http://www.scopus.com/inward/record.url?scp=77449138866&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77449138866&partnerID=8YFLogxK

U2 - 10.1002/cmmi.293

DO - 10.1002/cmmi.293

M3 - Article

C2 - 20101755

AN - SCOPUS:77449138866

SN - 1555-4309

VL - 5

SP - 34

EP - 38

JO - Contrast Media and Molecular Imaging

JF - Contrast Media and Molecular Imaging

IS - 1

ER -

Serine-derivatized gadonanotubes as magnetic nanoprobes for intracellular labeling

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this