Sex Genotyping Mice by Polymerase Chain Reaction

Virginia E. Papaioannou, Richard R. Behringer

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

A simple method to determine the genetic sex of a mouse is to amplify DNA from a male-specific gene by polymerase chain reaction (PCR). This protocol is used to detect the Y-chromosome-specific gene Sry in tissue lysates of tail tip or ear punch samples.

Original languageEnglish (US)
JournalCold Spring Harbor protocols
Volume2024
Issue number1
DOIs
StatePublished - Jan 2024
Externally publishedYes

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

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