TY - JOUR
T1 - Simple duplex fecal PCR assay that allows identification of false-negative results in Helicobacter sp.-infected mice
AU - Bourgade, Franck
AU - Montagutelli, Xavier
AU - Bigbee, Cassie
AU - Weiss, April
AU - Rigottier-Gois, Lionel
AU - Conti, Claudio J.
AU - Benavides, Fernando
PY - 2004/10
Y1 - 2004/10
N2 - We designed a simple and sensitive duplex polymerase chain reaction (PCR) assay for detection of false-negative results during routine Helicobacter sp. feces analysis. We took advantage of the various Lactobacillus species that form part of the normal intestinal flora of laboratory rodents to improve our PCR diagnostic assays. Using this one-step PCR assay, we were able to rule out false-negative results without the need of adding internal standard molecules. This is an important quality control for PCR diagnostic tests, since the presence of inhibitors in feces can prevent detection of Helicobacter infections using PCR analysis. Use of this Lactobacillus group-specific PCR assay can be extended to other feces tests used in mouse quality-control programs.
AB - We designed a simple and sensitive duplex polymerase chain reaction (PCR) assay for detection of false-negative results during routine Helicobacter sp. feces analysis. We took advantage of the various Lactobacillus species that form part of the normal intestinal flora of laboratory rodents to improve our PCR diagnostic assays. Using this one-step PCR assay, we were able to rule out false-negative results without the need of adding internal standard molecules. This is an important quality control for PCR diagnostic tests, since the presence of inhibitors in feces can prevent detection of Helicobacter infections using PCR analysis. Use of this Lactobacillus group-specific PCR assay can be extended to other feces tests used in mouse quality-control programs.
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UR - http://www.scopus.com/inward/citedby.url?scp=9344263477&partnerID=8YFLogxK
M3 - Article
C2 - 15575366
AN - SCOPUS:9344263477
SN - 1532-0820
VL - 54
SP - 528
EP - 532
JO - Comparative medicine
JF - Comparative medicine
IS - 5
ER -