TY - JOUR
T1 - Simultaneous molecular and hypoxia imaging of brain tumors in vivo using spectroscopic photoacoustic tomography
AU - Li, Meng Lin
AU - Oh, Jung Taek
AU - Xie, Xueyi
AU - Ku, Geng
AU - Wang, Wei
AU - Li, Chun
AU - Lungu, Gina
AU - Stoica, George
AU - Wang, Lihong V.
N1 - Funding Information:
Manuscript received June 1, 2007; revised September 5, 2007. This work was supported in part by the National Institutes of Health under Grants R01 EB000712 and R01 NS46214. M.-L. Li is with the Department of Electrical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan, R.O.C. J.-T. Oh and X. Xie are with the Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A&M University, College Station, TX 77843-3120 USA. G. Ku and L. V. Wang are with the Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO 63130-4899 USA (e-mail: lhwang@biomed.wustl.edu). W. Wang and C. Li are with the Department of Experimental Diagnostic Imaging, University of Texas M. D. Anderson Cancer Center, Houston, TX 77030 USA. G. Lungu and G. Stoica are with the Department of Veterinary Pathobiology, Texas A&M University, College Station, TX 77843-5547 USA.
PY - 2008/3
Y1 - 2008/3
N2 - Noninvasive molecular and functional imaging in vivo is promising for detecting and monitoring various physiological conditions in animals and ultimately humans. To this end, we present a novel noninvasive technology, spectroscopic photoacoustic tomography (SPAT), which offers both strong optical absorption contrast and high ultrasonic spatial resolution. Optical contrast allows spectroscopic separation of signal contributions from multiple optical absorbers (e.g., oxyhemoglobin, deoxyhemoglobin, and a molecular contrast agent), thus enabling simultaneous molecular and functional imaging. SPAT successfully imaged with high resolution the distribution of a molecular contrast agent targeting integrin overexpressed in human U87 glioblastomas in nude mouse brains. Simultaneously, SPAT also imaged the hemoglobin oxygen saturation and the total hemoglobin concentration of the vasculature, which revealed hypoxia in tumor neovasculature. Therefore, SPAT can potentially lead to better understanding of the interrelationships between hemodynamics and specific biomarkers associated with tumor progression.
AB - Noninvasive molecular and functional imaging in vivo is promising for detecting and monitoring various physiological conditions in animals and ultimately humans. To this end, we present a novel noninvasive technology, spectroscopic photoacoustic tomography (SPAT), which offers both strong optical absorption contrast and high ultrasonic spatial resolution. Optical contrast allows spectroscopic separation of signal contributions from multiple optical absorbers (e.g., oxyhemoglobin, deoxyhemoglobin, and a molecular contrast agent), thus enabling simultaneous molecular and functional imaging. SPAT successfully imaged with high resolution the distribution of a molecular contrast agent targeting integrin overexpressed in human U87 glioblastomas in nude mouse brains. Simultaneously, SPAT also imaged the hemoglobin oxygen saturation and the total hemoglobin concentration of the vasculature, which revealed hypoxia in tumor neovasculature. Therefore, SPAT can potentially lead to better understanding of the interrelationships between hemodynamics and specific biomarkers associated with tumor progression.
KW - Functional imaging
KW - Hemoglobin oxygen saturation
KW - Molecular imaging
KW - Spectroscopic photoacoustic tomography
KW - Total hemoglobin concentration
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U2 - 10.1109/JPROC.2007.913515
DO - 10.1109/JPROC.2007.913515
M3 - Article
AN - SCOPUS:42149148395
SN - 0018-9219
VL - 96
SP - 481
EP - 489
JO - Proceedings of the IEEE
JF - Proceedings of the IEEE
IS - 3
M1 - 4445026
ER -