Single-cell quantitative PCR: Advances and potential in cancer diagnostics

Chi Young Ok, Rajesh R Singh, Alaa A. Salim

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Tissues are heterogeneous in their components. If cells of interest are a minor population of collected tissue, it would be difficult to obtain genetic or genomic information of the interested cell population with conventional genomic DNA extraction from the collected tissue. Single-cell DNA analysis is important in the analysis of genetics of cell clonality, genetic anticipation, and single-cell DNA polymorphisms. Single- cell PCR using Single Cell Ampligrid/GeXP platform is described in this chapter.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages153-159
Number of pages7
DOIs
StatePublished - Feb 1 2016

Publication series

NameMethods in Molecular Biology
Volume1392
ISSN (Print)1064-3745

Keywords

  • Cancer
  • Diagnostics
  • Quantitative
  • RT-PCR
  • Single-cell analysis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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