@article{2ea44e51a33f4d6389b904c53ca5e2be,
title = "Single-Cell RNA-Seq Reveals Dynamic Early Embryonic-like Programs during Chemical Reprogramming",
abstract = "Single-cell RNA sequencing analysis of chemical reprogramming depicts its trajectory and highlights dynamic intermediate cellular programs resembling early embryonic signatures. Zhao et al. apply these insights to develop a faster reprogramming system.",
keywords = "2C-like program, CiPSC, XEN-like cell, Zscan4, chemical reprogramming, early embryonic-like programs, genome-wide hypomethylation, pluripotency, reprogramming trajectory, single-cell RNA-seq",
author = "Ting Zhao and Yao Fu and Jialiang Zhu and Yifang Liu and Qian Zhang and Zexuan Yi and Shi Chen and Zhonggang Jiao and Xiaochan Xu and Junquan Xu and Shuguang Duo and Yun Bai and Chao Tang and Cheng Li and Hongkui Deng",
note = "Funding Information: We would like to thank Dr. Minoru Ko for the gift of p Zscan4c -Emerald ESCs. We thank the members of the Deng lab for the discussion of the manuscript, including Jun Xu, Jingyang Guan, Jinlin Wang, Gaofan Meng, Yanqin Li, Junqing Ye, Xu Zhang, and others. We thank Weifeng Yang, Xuefang Zhang, Liying Du, Xiaochen Li, Hongxia Lv, Yaqin Du, Ting Wang, and Chaoran Zhao for technical assistance; the Core Facilities at School of Life Sciences and Joint Center for Life Sciences Public Platform, Peking University for the assistance with confocal microscopy and cell sorting; and the High-Performance Computing Platform of the Center for Life Science, Peking University and National Supercomputer Center in Guangzhou (NSCC-GZ) TianHe-2 for the support of bioinformatics calculations. This work was supported by the National Key Research and Development Program of China ( 2016YFA0100103 and 2017YFA0103000 ), the National Natural Science Foundation of China ( 31730059 and 31521004 ), the Guangdong Innovative and Entrepreneurial Research Team Program ( 2014ZT05S216 ), the Science and Technology Planning Project of Guangdong Province, China ( 2014B020226001 and 2016B030232001 ), and the Science and Technology Program of Guangzhou, China ( 201508020001 ). This work was supported in part by a grant from the BeiHao Stem Cell and Regenerative Medicine Translational Research Institute . S. D. was supported by the CAS Key Technology Talent Program. Funding Information: We would like to thank Dr. Minoru Ko for the gift of pZscan4c-Emerald ESCs. We thank the members of the Deng lab for the discussion of the manuscript, including Jun Xu, Jingyang Guan, Jinlin Wang, Gaofan Meng, Yanqin Li, Junqing Ye, Xu Zhang, and others. We thank Weifeng Yang, Xuefang Zhang, Liying Du, Xiaochen Li, Hongxia Lv, Yaqin Du, Ting Wang, and Chaoran Zhao for technical assistance; the Core Facilities at School of Life Sciences and Joint Center for Life Sciences Public Platform, Peking University for the assistance with confocal microscopy and cell sorting; and the High-Performance Computing Platform of the Center for Life Science, Peking University and National Supercomputer Center in Guangzhou (NSCC-GZ) TianHe-2 for the support of bioinformatics calculations. This work was supported by the National Key Research and Development Program of China (2016YFA0100103 and 2017YFA0103000), the National Natural Science Foundation of China (31730059 and 31521004), the Guangdong Innovative and Entrepreneurial Research Team Program (2014ZT05S216), the Science and Technology Planning Project of Guangdong Province, China (2014B020226001 and 2016B030232001), and the Science and Technology Program of Guangzhou, China (201508020001). This work was supported in part by a grant from the BeiHao Stem Cell and Regenerative Medicine Translational Research Institute. S. D. was supported by the CAS Key Technology Talent Program.",
year = "2018",
month = jul,
day = "5",
doi = "10.1016/j.stem.2018.05.025",
language = "English (US)",
volume = "23",
pages = "31--45.e7",
journal = "Cell Stem Cell",
issn = "1934-5909",
publisher = "Cell Press",
number = "1",
}