@inproceedings{deb75cc531184d5d88c51f6a4ce331c9,
title = "SiRNA for therapeutic immuno-modulation: Simultaneous delivery of cytokine targeted SiRNA and DNA antigens to dendritic cells using polymer microcarriers",
abstract = "RNA interference mediated gene knockdown is a potential immuno-modulatory tool for tuning dendritic cells (DC) activation and function in-vivo. We have developed a novel multi-tiered delivery system for combinatorial administration of siRNA and plasmid DNA antigens in a single gene carrier system. Our hypothesis is that codelivery of IL-10 specific siRNA along with DNA antigens to the same dendritic cells would inhibit IL-10 production, enhance DC activation, antigen presentation, and T cell response. Non-toxic, polyethyleneimine (PEI)-functionalized, poly(lactic-co-glycolic acid) (PEI-PLGA) microparticles co-delivering IL-10 siRNA and DNA antigen exhibited significantly higher (p<0.05) IL-10 gene knockdown in primary DCs, and DNA transfection, invitro. Mice immunized intramuscularly with (IL-10)-SiRNA-HBsAg-PEI-PLGA particles demonstrated successful {"}switch{"} towards a stronger Th1 response as compared to naked DNA or HBsAg loaded PEI-PLGA treated animals.",
keywords = "Combinatorial delivery, Immuno-modulation, Interleukin-10, siRNA, Th1 response",
author = "Ankur Singh and Hui Nie and Bilal Ghosn and Krishnendu Roy",
year = "2008",
language = "English (US)",
isbn = "9781420085075",
series = "Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008",
pages = "350--353",
booktitle = "Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008",
note = "2008 NSTI Nanotechnology Conference and Trade Show, NSTI Nanotech 2008 Joint Meeting, Nanotechnology 2008 ; Conference date: 01-06-2008 Through 05-06-2008",
}