Specific association between the human DNA repair proteins XPA and ERCC1

Lei Li; Stephen J. Elledge; Carolyn A. Peterson; Elise S. Bales; Randy J. Legerski

doi:10.1073/pnas.91.11.5012

Specific association between the human DNA repair proteins XPA and ERCC1

Lei Li, Stephen J. Elledge, Carolyn A. Peterson, Elise S. Bales, Randy J. Legerski

Research output: Contribution to journal › Article › peer-review

279 Scopus citations

Abstract

Processing of DNA damage by the nucleotide-excision repair pathway in eukaryotic cells is most likely accomplished by multiprotein complexes. However, the nature of these complexes and the details of the molecular interactions between DNA repair factors are for the most part unknown. Here, we demonstrate both in vivo, using the two-hybrid system, and in vitro, using recombinant proteins, that the human repair factors XPA and ERCC1 specifically interact. In addition, we report an initial determination of the domains in ERCC1 and XPA that mediate this interaction. These results suggest that XPA may play a role in the localization or loading of an incision complex, composed of ERCC1 and possibly other repair factors, onto a damaged site.

Original language	English (US)
Pages (from-to)	5012-5016
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	91
Issue number	11
DOIs	https://doi.org/10.1073/pnas.91.11.5012
State	Published - May 24 1994

Keywords

nucleotide excision repair
protein-protein interaction
xeroderma pigmentosum

ASJC Scopus subject areas

General

Access to Document

10.1073/pnas.91.11.5012

Cite this

@article{66d05929ef464e5aa9ff4ba8b53cf292,

title = "Specific association between the human DNA repair proteins XPA and ERCC1",

abstract = "Processing of DNA damage by the nucleotide-excision repair pathway in eukaryotic cells is most likely accomplished by multiprotein complexes. However, the nature of these complexes and the details of the molecular interactions between DNA repair factors are for the most part unknown. Here, we demonstrate both in vivo, using the two-hybrid system, and in vitro, using recombinant proteins, that the human repair factors XPA and ERCC1 specifically interact. In addition, we report an initial determination of the domains in ERCC1 and XPA that mediate this interaction. These results suggest that XPA may play a role in the localization or loading of an incision complex, composed of ERCC1 and possibly other repair factors, onto a damaged site.",

keywords = "nucleotide excision repair, protein-protein interaction, xeroderma pigmentosum",

author = "Lei Li and Elledge, {Stephen J.} and Peterson, {Carolyn A.} and Bales, {Elise S.} and Legerski, {Randy J.}",

year = "1994",

month = may,

day = "24",

doi = "10.1073/pnas.91.11.5012",

language = "English (US)",

volume = "91",

pages = "5012--5016",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "11",

}

TY - JOUR

T1 - Specific association between the human DNA repair proteins XPA and ERCC1

AU - Li, Lei

AU - Elledge, Stephen J.

AU - Peterson, Carolyn A.

AU - Bales, Elise S.

AU - Legerski, Randy J.

PY - 1994/5/24

Y1 - 1994/5/24

N2 - Processing of DNA damage by the nucleotide-excision repair pathway in eukaryotic cells is most likely accomplished by multiprotein complexes. However, the nature of these complexes and the details of the molecular interactions between DNA repair factors are for the most part unknown. Here, we demonstrate both in vivo, using the two-hybrid system, and in vitro, using recombinant proteins, that the human repair factors XPA and ERCC1 specifically interact. In addition, we report an initial determination of the domains in ERCC1 and XPA that mediate this interaction. These results suggest that XPA may play a role in the localization or loading of an incision complex, composed of ERCC1 and possibly other repair factors, onto a damaged site.

AB - Processing of DNA damage by the nucleotide-excision repair pathway in eukaryotic cells is most likely accomplished by multiprotein complexes. However, the nature of these complexes and the details of the molecular interactions between DNA repair factors are for the most part unknown. Here, we demonstrate both in vivo, using the two-hybrid system, and in vitro, using recombinant proteins, that the human repair factors XPA and ERCC1 specifically interact. In addition, we report an initial determination of the domains in ERCC1 and XPA that mediate this interaction. These results suggest that XPA may play a role in the localization or loading of an incision complex, composed of ERCC1 and possibly other repair factors, onto a damaged site.

KW - nucleotide excision repair

KW - protein-protein interaction

KW - xeroderma pigmentosum

UR - http://www.scopus.com/inward/record.url?scp=0028276805&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028276805&partnerID=8YFLogxK

U2 - 10.1073/pnas.91.11.5012

DO - 10.1073/pnas.91.11.5012

M3 - Article

C2 - 8197174

AN - SCOPUS:0028276805

SN - 0027-8424

VL - 91

SP - 5012

EP - 5016

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 11

ER -

Specific association between the human DNA repair proteins XPA and ERCC1

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this