Spinal glial glutamate transporters downregulate in rats with taxol-induced hyperalgesia

Han Rong Weng; Natarajan Aravindan; Juan P. Cata; Jing Hong Chen; Andrew D.S. Shaw; Patrick M. Dougherty

doi:10.1016/j.neulet.2005.05.049

Spinal glial glutamate transporters downregulate in rats with taxol-induced hyperalgesia

Han Rong Weng, Natarajan Aravindan, Juan P. Cata, Jing Hong Chen, Andrew D.S. Shaw, Patrick M. Dougherty

Research output: Contribution to journal › Article › peer-review

92 Scopus citations

Abstract

Changes in the expression of glial glutamate transporters (GLAST and GLT-1) were examined in the spinal cord of rats with chemotherapy (taxol)-induced mechanical hyperalgesia. Immunohistochemical studies show that the expression of both GLAST and GLT-1 in the L4-L5 spinal dorsal horn is decreased by 24% (P < 0.001) and 23% (P < 0.001), respectively, in rats with taxol-induced hyperalgesia as compared with those in control rats. These changes were further confirmed using an enzyme-linked immunosorbent assay that confirmed downregulation of GLAST by 36% (P < 0.05) and GLT-1 by 18% (P < 0.05) in the L4-L5 spinal cord of taxol-treated rats. These data indicate that downregulation of glutamate transporters may contribute to the development of hyperalgesia induced by taxol and suggest that glutamate transporters may be a new target for treatment of pain.

Original language	English (US)
Pages (from-to)	18-22
Number of pages	5
Journal	Neuroscience Letters
Volume	386
Issue number	1
DOIs	https://doi.org/10.1016/j.neulet.2005.05.049
State	Published - Sep 23 2005

Keywords

Astrocytes
Chemotherapy
GLAST
GLT-1
Glutamate transporters
Hyperalgesia
Paclitaxel
Pain

ASJC Scopus subject areas

General Neuroscience

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10.1016/j.neulet.2005.05.049

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@article{c667d33a7c7f4680b1a79d5fa7a9199b,

title = "Spinal glial glutamate transporters downregulate in rats with taxol-induced hyperalgesia",

abstract = "Changes in the expression of glial glutamate transporters (GLAST and GLT-1) were examined in the spinal cord of rats with chemotherapy (taxol)-induced mechanical hyperalgesia. Immunohistochemical studies show that the expression of both GLAST and GLT-1 in the L4-L5 spinal dorsal horn is decreased by 24% (P < 0.001) and 23% (P < 0.001), respectively, in rats with taxol-induced hyperalgesia as compared with those in control rats. These changes were further confirmed using an enzyme-linked immunosorbent assay that confirmed downregulation of GLAST by 36% (P < 0.05) and GLT-1 by 18% (P < 0.05) in the L4-L5 spinal cord of taxol-treated rats. These data indicate that downregulation of glutamate transporters may contribute to the development of hyperalgesia induced by taxol and suggest that glutamate transporters may be a new target for treatment of pain.",

keywords = "Astrocytes, Chemotherapy, GLAST, GLT-1, Glutamate transporters, Hyperalgesia, Paclitaxel, Pain",

author = "Weng, {Han Rong} and Natarajan Aravindan and Cata, {Juan P.} and Chen, {Jing Hong} and Shaw, {Andrew D.S.} and Dougherty, {Patrick M.}",

note = "Funding Information: This work was supported by NIH award NS-39933 and funds provided by the University of Texas M.D. Anderson Cancer Center.",

year = "2005",

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doi = "10.1016/j.neulet.2005.05.049",

language = "English (US)",

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AU - Weng, Han Rong

AU - Aravindan, Natarajan

AU - Cata, Juan P.

AU - Chen, Jing Hong

AU - Shaw, Andrew D.S.

AU - Dougherty, Patrick M.

N1 - Funding Information: This work was supported by NIH award NS-39933 and funds provided by the University of Texas M.D. Anderson Cancer Center.

PY - 2005/9/23

Y1 - 2005/9/23

N2 - Changes in the expression of glial glutamate transporters (GLAST and GLT-1) were examined in the spinal cord of rats with chemotherapy (taxol)-induced mechanical hyperalgesia. Immunohistochemical studies show that the expression of both GLAST and GLT-1 in the L4-L5 spinal dorsal horn is decreased by 24% (P < 0.001) and 23% (P < 0.001), respectively, in rats with taxol-induced hyperalgesia as compared with those in control rats. These changes were further confirmed using an enzyme-linked immunosorbent assay that confirmed downregulation of GLAST by 36% (P < 0.05) and GLT-1 by 18% (P < 0.05) in the L4-L5 spinal cord of taxol-treated rats. These data indicate that downregulation of glutamate transporters may contribute to the development of hyperalgesia induced by taxol and suggest that glutamate transporters may be a new target for treatment of pain.

AB - Changes in the expression of glial glutamate transporters (GLAST and GLT-1) were examined in the spinal cord of rats with chemotherapy (taxol)-induced mechanical hyperalgesia. Immunohistochemical studies show that the expression of both GLAST and GLT-1 in the L4-L5 spinal dorsal horn is decreased by 24% (P < 0.001) and 23% (P < 0.001), respectively, in rats with taxol-induced hyperalgesia as compared with those in control rats. These changes were further confirmed using an enzyme-linked immunosorbent assay that confirmed downregulation of GLAST by 36% (P < 0.05) and GLT-1 by 18% (P < 0.05) in the L4-L5 spinal cord of taxol-treated rats. These data indicate that downregulation of glutamate transporters may contribute to the development of hyperalgesia induced by taxol and suggest that glutamate transporters may be a new target for treatment of pain.

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KW - Chemotherapy

KW - GLAST

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KW - Paclitaxel

KW - Pain

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Spinal glial glutamate transporters downregulate in rats with taxol-induced hyperalgesia

Abstract

Keywords

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