TY - JOUR
T1 - Spying on cancer
T2 - Molecular imaging in vivo with genetically encoded reporters
AU - Gross, Shimon
AU - Piwnica-Worms, David
N1 - Funding Information:
The authors would like to thank colleagues in the Washington University Molecular Imaging Center for their insightful discussions contributing to this review. Supported by NIH P50 CA94056.
PY - 2005/1
Y1 - 2005/1
N2 - Genetically encoded imaging reporters introduced into cells and transgenic animals enable noninvasive, longitudinal studies of dynamic biological processes in vivo. The most common reporters include firefly luciferase (bioluminescence imaging), green fluorescence protein (fluorescence imaging), herpes simplex virus-1 thymidine kinase (positron emission tomography), and variants with enhanced spectral and kinetic properties. When cloned into promoter/enhancer sequences or engineered into fusion proteins, imaging reporters allow transcriptional regulation, signal transduction, protein-protein interactions, oncogenic transformation, cell trafficking, and targeted drug action to be spatiotemporally resolved in vivo. Spying on cancer with genetically encoded imaging reporters provides insight into cancer-specific molecular machinery within the context of the whole animal.
AB - Genetically encoded imaging reporters introduced into cells and transgenic animals enable noninvasive, longitudinal studies of dynamic biological processes in vivo. The most common reporters include firefly luciferase (bioluminescence imaging), green fluorescence protein (fluorescence imaging), herpes simplex virus-1 thymidine kinase (positron emission tomography), and variants with enhanced spectral and kinetic properties. When cloned into promoter/enhancer sequences or engineered into fusion proteins, imaging reporters allow transcriptional regulation, signal transduction, protein-protein interactions, oncogenic transformation, cell trafficking, and targeted drug action to be spatiotemporally resolved in vivo. Spying on cancer with genetically encoded imaging reporters provides insight into cancer-specific molecular machinery within the context of the whole animal.
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U2 - 10.1016/j.ccr.2004.12.011
DO - 10.1016/j.ccr.2004.12.011
M3 - Review article
C2 - 15652745
AN - SCOPUS:12344300543
SN - 1535-6108
VL - 7
SP - 5
EP - 15
JO - Cancer cell
JF - Cancer cell
IS - 1
ER -