Abstract
Background: We have previously shown reversal of E-cadherin methylation in gastric mucosa from patients with dyspepsia at week 6 after Helicobacter pylori-eradication therapy. But the long-term methylation status of these patients was unknown. Aim: To investigate the methylation status at E-cadherin at year 3 after H. pylori-eradication therapy. Methods: 35 patients (25 with and 10 without H. pylori eradicated) enrolled in our previous study were recruited into the present study (year 3 analysis). Methylation at E-cadherin was evaluated by methylation-specific polymerase chain reaction method. Results: There was no difference in age and sex distribution in the two groups. Methylation at E-cadherin in patients with H. pylori eradicated at weeks 0, 6 and year 3 were 52%, 20% and 20%, respectively. Concordant methylation status at week 6 and year 3 was 92%. Methylation at E-cadherin in patients without H. pylori at weeks 0, 6 and year 3 were 50%, 60% and 60%, respectively. Concordant methylation status between week 6 and year 3 was 90%. Stability of E-cadherin methylation status was associated with histological changes. No association between E-cadherin methylation status and age was observed. Conclusion: The methylation pattern is stable for a long period, thus suggesting the effect of environment on methylation.
Original language | English (US) |
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Pages (from-to) | 831-836 |
Number of pages | 6 |
Journal | Alimentary Pharmacology and Therapeutics |
Volume | 24 |
Issue number | 5 |
DOIs | |
State | Published - Sep 2006 |
ASJC Scopus subject areas
- Hepatology
- Gastroenterology
- Pharmacology (medical)