Structural basis for norovirus inhibition by human milk oligosaccharides

Stefan Weichert; Anna Koromyslova; Bishal K. Singh; Satoko Hansman; Stefan Jennewein; Horst Schroten; Grant S. Hansman

doi:10.1128/JVI.03223-15

Structural basis for norovirus inhibition by human milk oligosaccharides

Stefan Weichert, Anna Koromyslova, Bishal K. Singh, Satoko Hansman, Stefan Jennewein, Horst Schroten, Grant S. Hansman

Research output: Contribution to journal › Article › peer-review

110 Scopus citations

Abstract

Histo-blood group antigens (HBGAs) are important binding factors for norovirus infections. We show that two human milk oligosaccharides, 2'-fucosyllactose (2'FL) and 3-fucosyllactose (3FL), could block norovirus from binding to surrogate HBGA samples. We found that 2'FL and 3FL bound at the equivalent HBGA pockets on the norovirus capsid using X-ray crystallography. Our data revealed that 2'FL and 3FL structurally mimic HBGAs. These results suggest that 2'FL and 3FL might act as naturally occurring decoys in humans.

Original language	English (US)
Pages (from-to)	4843-4848
Number of pages	6
Journal	Journal of Virology
Volume	90
Issue number	9
DOIs	https://doi.org/10.1128/JVI.03223-15
State	Published - May 1 2016
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Immunology
Insect Science
Virology

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abstract = "Histo-blood group antigens (HBGAs) are important binding factors for norovirus infections. We show that two human milk oligosaccharides, 2'-fucosyllactose (2'FL) and 3-fucosyllactose (3FL), could block norovirus from binding to surrogate HBGA samples. We found that 2'FL and 3FL bound at the equivalent HBGA pockets on the norovirus capsid using X-ray crystallography. Our data revealed that 2'FL and 3FL structurally mimic HBGAs. These results suggest that 2'FL and 3FL might act as naturally occurring decoys in humans.",

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T1 - Structural basis for norovirus inhibition by human milk oligosaccharides

AU - Weichert, Stefan

AU - Koromyslova, Anna

AU - Singh, Bishal K.

AU - Hansman, Satoko

AU - Jennewein, Stefan

AU - Schroten, Horst

AU - Hansman, Grant S.

PY - 2016/5/1

Y1 - 2016/5/1

N2 - Histo-blood group antigens (HBGAs) are important binding factors for norovirus infections. We show that two human milk oligosaccharides, 2'-fucosyllactose (2'FL) and 3-fucosyllactose (3FL), could block norovirus from binding to surrogate HBGA samples. We found that 2'FL and 3FL bound at the equivalent HBGA pockets on the norovirus capsid using X-ray crystallography. Our data revealed that 2'FL and 3FL structurally mimic HBGAs. These results suggest that 2'FL and 3FL might act as naturally occurring decoys in humans.

AB - Histo-blood group antigens (HBGAs) are important binding factors for norovirus infections. We show that two human milk oligosaccharides, 2'-fucosyllactose (2'FL) and 3-fucosyllactose (3FL), could block norovirus from binding to surrogate HBGA samples. We found that 2'FL and 3FL bound at the equivalent HBGA pockets on the norovirus capsid using X-ray crystallography. Our data revealed that 2'FL and 3FL structurally mimic HBGAs. These results suggest that 2'FL and 3FL might act as naturally occurring decoys in humans.

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Structural basis for norovirus inhibition by human milk oligosaccharides

Abstract

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