Study of Immunosuppressive Activity of a Synthetic Decapeptide Corresponding to an ACTH-Like Sequence of Human Immunoglobulin G1

E. V. Navolotskaya; T. A. Zargarova; T. N. Lepikhova; V. I. Turobov; R. I. Nurieva; N. V. Malkova; V. M. Lipkin; V. P. Zav'yalov

Study of Immunosuppressive Activity of a Synthetic Decapeptide Corresponding to an ACTH-Like Sequence of Human Immunoglobulin G1

E. V. Navolotskaya, T. A. Zargarova, T. N. Lepikhova, V. I. Turobov, R. I. Nurieva, N. V. Malkova, V. M. Lipkin, V. P. Zav'yalov

Research output: Contribution to journal › Review article › peer-review

5 Scopus citations

Abstract

The synthetic ACTH-like decapeptide H-Val-Lys-Lys-Pro-Gly- Ser-Ser-Val-Lys-Val-OH, corresponding to amino acid residues 11-20 of the variable part of the human IgG1 heavy chain (referred to as immunocortin) was found to have an immunosuppressive effect on cells in vitro: it inhibits blast transformation of mouse thymocytes and reduces spontaneous motility of mouse peritoneal macrophages as well as their bactericidal activity against the virulent bacterial strain Salmonella typhimurium 415. Tritium-labeled immunocortin binds with high affinity to ACTH receptors on thymocytes and macrophages (K_d 2.1 and 2.5 nM, respectively) and activates adenylate cyclase in these cells. Thus, the interaction of immunocortin with the target cell includes the following main steps: binding to the receptor, activation of adenylate cyclase, and elevation of the intracellular content of cAMP.

Original language	English (US)
Pages (from-to)	758-764
Number of pages	7
Journal	Biochemistry (Moscow)
Volume	64
Issue number	7
State	Published - Jul 1999
Externally published	Yes

Keywords

Adenylate cyclase
Adrenocorticotropic hormone (ACTH)
Immune system
Immunoglobulin G (IgG)
Peptides
Peritoneal macrophages
Receptors
Thymocytes

ASJC Scopus subject areas

Biochemistry

Cite this

@article{20a5660d699742bf9a205f68d43692fe,

title = "Study of Immunosuppressive Activity of a Synthetic Decapeptide Corresponding to an ACTH-Like Sequence of Human Immunoglobulin G1",

abstract = "The synthetic ACTH-like decapeptide H-Val-Lys-Lys-Pro-Gly- Ser-Ser-Val-Lys-Val-OH, corresponding to amino acid residues 11-20 of the variable part of the human IgG1 heavy chain (referred to as immunocortin) was found to have an immunosuppressive effect on cells in vitro: it inhibits blast transformation of mouse thymocytes and reduces spontaneous motility of mouse peritoneal macrophages as well as their bactericidal activity against the virulent bacterial strain Salmonella typhimurium 415. Tritium-labeled immunocortin binds with high affinity to ACTH receptors on thymocytes and macrophages (Kd 2.1 and 2.5 nM, respectively) and activates adenylate cyclase in these cells. Thus, the interaction of immunocortin with the target cell includes the following main steps: binding to the receptor, activation of adenylate cyclase, and elevation of the intracellular content of cAMP.",

keywords = "Adenylate cyclase, Adrenocorticotropic hormone (ACTH), Immune system, Immunoglobulin G (IgG), Peptides, Peritoneal macrophages, Receptors, Thymocytes",

author = "Navolotskaya, {E. V.} and Zargarova, {T. A.} and Lepikhova, {T. N.} and Turobov, {V. I.} and Nurieva, {R. I.} and Malkova, {N. V.} and Lipkin, {V. M.} and Zav'yalov, {V. P.}",

year = "1999",

month = jul,

language = "English (US)",

volume = "64",

pages = "758--764",

journal = "Biochemistry (Moscow)",

issn = "0006-2979",

publisher = "Maik Nauka-Interperiodica Publishing",

number = "7",

}

TY - JOUR

T1 - Study of Immunosuppressive Activity of a Synthetic Decapeptide Corresponding to an ACTH-Like Sequence of Human Immunoglobulin G1

AU - Navolotskaya, E. V.

AU - Zargarova, T. A.

AU - Lepikhova, T. N.

AU - Turobov, V. I.

AU - Nurieva, R. I.

AU - Malkova, N. V.

AU - Lipkin, V. M.

AU - Zav'yalov, V. P.

PY - 1999/7

Y1 - 1999/7

N2 - The synthetic ACTH-like decapeptide H-Val-Lys-Lys-Pro-Gly- Ser-Ser-Val-Lys-Val-OH, corresponding to amino acid residues 11-20 of the variable part of the human IgG1 heavy chain (referred to as immunocortin) was found to have an immunosuppressive effect on cells in vitro: it inhibits blast transformation of mouse thymocytes and reduces spontaneous motility of mouse peritoneal macrophages as well as their bactericidal activity against the virulent bacterial strain Salmonella typhimurium 415. Tritium-labeled immunocortin binds with high affinity to ACTH receptors on thymocytes and macrophages (Kd 2.1 and 2.5 nM, respectively) and activates adenylate cyclase in these cells. Thus, the interaction of immunocortin with the target cell includes the following main steps: binding to the receptor, activation of adenylate cyclase, and elevation of the intracellular content of cAMP.

AB - The synthetic ACTH-like decapeptide H-Val-Lys-Lys-Pro-Gly- Ser-Ser-Val-Lys-Val-OH, corresponding to amino acid residues 11-20 of the variable part of the human IgG1 heavy chain (referred to as immunocortin) was found to have an immunosuppressive effect on cells in vitro: it inhibits blast transformation of mouse thymocytes and reduces spontaneous motility of mouse peritoneal macrophages as well as their bactericidal activity against the virulent bacterial strain Salmonella typhimurium 415. Tritium-labeled immunocortin binds with high affinity to ACTH receptors on thymocytes and macrophages (Kd 2.1 and 2.5 nM, respectively) and activates adenylate cyclase in these cells. Thus, the interaction of immunocortin with the target cell includes the following main steps: binding to the receptor, activation of adenylate cyclase, and elevation of the intracellular content of cAMP.

KW - Adenylate cyclase

KW - Adrenocorticotropic hormone (ACTH)

KW - Immune system

KW - Immunoglobulin G (IgG)

KW - Peptides

KW - Peritoneal macrophages

KW - Receptors

KW - Thymocytes

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UR - http://www.scopus.com/inward/citedby.url?scp=0033159027&partnerID=8YFLogxK

M3 - Review article

C2 - 10424898

AN - SCOPUS:0033159027

SN - 0006-2979

VL - 64

SP - 758

EP - 764

JO - Biochemistry (Moscow)

JF - Biochemistry (Moscow)

IS - 7

ER -

Study of Immunosuppressive Activity of a Synthetic Decapeptide Corresponding to an ACTH-Like Sequence of Human Immunoglobulin G1

Abstract

Keywords

ASJC Scopus subject areas

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