Study of interaction between L-glutamate and human blood lymphocytes

I. A. Kostanyan; M. I. Merkulova; E. V. Navolotskaya; R. I. Nurieva

doi:10.1016/S0165-2478(97)00086-2

Study of interaction between L-glutamate and human blood lymphocytes

I. A. Kostanyan, M. I. Merkulova, E. V. Navolotskaya, R. I. Nurieva

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

The presence of specific glutamate receptors on T lymphocytes separated from normal human blood was investigated. Upon study of T lymphocytes interaction with [³H]glutamate the last one was found to bind specifically to these cells, K(d) = 2.36 x 10^-7 M. Thirteen unlabelled compounds were tested as potential glutamate competitors. It was shown that only quisqualate (a structural analogue of glutamate) and dipeptides Ala-Glu, Glu-Ala, Glu- Glu competited with [³H]glutamate in binding to a common binding site. The experiments on binding of labelled and unlabelled glutamate bulky conjugates with dextran have demonstrated that both conjugated and free glutamate interacted with the common binding sites on the outer cell membrane. By this means it was shown that T lymphocytes exposed quisqualate-sensitive glutamate receptors on the outer surface of the plasma membrane.

Original language	English (US)
Pages (from-to)	177-180
Number of pages	4
Journal	Immunology Letters
Volume	58
Issue number	3
DOIs	https://doi.org/10.1016/S0165-2478(97)00086-2
State	Published - Aug 1997
Externally published	Yes

Keywords

Glutamate receptors
Human T lymphocytes
L-Glutamate
Synthetic peptides

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/S0165-2478(97)00086-2

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title = "Study of interaction between L-glutamate and human blood lymphocytes",

abstract = "The presence of specific glutamate receptors on T lymphocytes separated from normal human blood was investigated. Upon study of T lymphocytes interaction with [3H]glutamate the last one was found to bind specifically to these cells, K(d) = 2.36 x 10-7 M. Thirteen unlabelled compounds were tested as potential glutamate competitors. It was shown that only quisqualate (a structural analogue of glutamate) and dipeptides Ala-Glu, Glu-Ala, Glu- Glu competited with [3H]glutamate in binding to a common binding site. The experiments on binding of labelled and unlabelled glutamate bulky conjugates with dextran have demonstrated that both conjugated and free glutamate interacted with the common binding sites on the outer cell membrane. By this means it was shown that T lymphocytes exposed quisqualate-sensitive glutamate receptors on the outer surface of the plasma membrane.",

keywords = "Glutamate receptors, Human T lymphocytes, L-Glutamate, Synthetic peptides",

author = "Kostanyan, {I. A.} and Merkulova, {M. I.} and Navolotskaya, {E. V.} and Nurieva, {R. I.}",

note = "Funding Information: The work was supported by Grant (No. 95-04-13284a) of the Russian Foundation of Basic Research. ",

year = "1997",

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AU - Kostanyan, I. A.

AU - Merkulova, M. I.

AU - Navolotskaya, E. V.

AU - Nurieva, R. I.

N1 - Funding Information: The work was supported by Grant (No. 95-04-13284a) of the Russian Foundation of Basic Research.

PY - 1997/8

Y1 - 1997/8

N2 - The presence of specific glutamate receptors on T lymphocytes separated from normal human blood was investigated. Upon study of T lymphocytes interaction with [3H]glutamate the last one was found to bind specifically to these cells, K(d) = 2.36 x 10-7 M. Thirteen unlabelled compounds were tested as potential glutamate competitors. It was shown that only quisqualate (a structural analogue of glutamate) and dipeptides Ala-Glu, Glu-Ala, Glu- Glu competited with [3H]glutamate in binding to a common binding site. The experiments on binding of labelled and unlabelled glutamate bulky conjugates with dextran have demonstrated that both conjugated and free glutamate interacted with the common binding sites on the outer cell membrane. By this means it was shown that T lymphocytes exposed quisqualate-sensitive glutamate receptors on the outer surface of the plasma membrane.

AB - The presence of specific glutamate receptors on T lymphocytes separated from normal human blood was investigated. Upon study of T lymphocytes interaction with [3H]glutamate the last one was found to bind specifically to these cells, K(d) = 2.36 x 10-7 M. Thirteen unlabelled compounds were tested as potential glutamate competitors. It was shown that only quisqualate (a structural analogue of glutamate) and dipeptides Ala-Glu, Glu-Ala, Glu- Glu competited with [3H]glutamate in binding to a common binding site. The experiments on binding of labelled and unlabelled glutamate bulky conjugates with dextran have demonstrated that both conjugated and free glutamate interacted with the common binding sites on the outer cell membrane. By this means it was shown that T lymphocytes exposed quisqualate-sensitive glutamate receptors on the outer surface of the plasma membrane.

KW - Glutamate receptors

KW - Human T lymphocytes

KW - L-Glutamate

KW - Synthetic peptides

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SP - 177

EP - 180

JO - Immunology Letters

JF - Immunology Letters

IS - 3

ER -

Study of interaction between L-glutamate and human blood lymphocytes

Abstract

Keywords

ASJC Scopus subject areas

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