Abstract
Purpose. DNA markers are useful in assessing cell proliferation. The purpose of this study was to synthesize 99mTc-ethylenedicysteine- guanine (EC-Guan) for evaluation of cell proliferation. Methods. Tumor cells were incubated with 99mTc-EC-Guan for cell cycle analysis. Prostate tumor cells that were overexpressing the HSV thymidine kinase gene, or various tumor cells were incubated with 99mTc-EC-Guan at 0.5-2 h. Thymidine incorporation assays were performed in lung cancer cells incubated with EC-Guan at 0.1-1 mg/well. Tissue distribution, autoradiography, and planar scintigraphy of 99mTc-EC-Guan and 99mTc-EC (control) were determined in tumor-bearing rodents at 0.5-4 h. Results. Cell culture assays indicated that EC-Guan was incorporated in DNA, and there was no significant uptake difference between HSVTK overexpressed and normal groups. Biodistribution and scintigraphic imaging studies of 99mTc-EC-Guan showed increased tumor/tissue count density ratios as a function of time. Conclusions. Our results indicate that 99mTc-EC-Guan may be useful as a tumor proliferation imaging agent.
Original language | English (US) |
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Pages (from-to) | 1471-1479 |
Number of pages | 9 |
Journal | Pharmaceutical Research |
Volume | 22 |
Issue number | 9 |
DOIs | |
State | Published - Sep 2005 |
Keywords
- Biodistribution
- EC-Guan
- Proliferation
- SPECT
ASJC Scopus subject areas
- Biotechnology
- Molecular Medicine
- Pharmacology
- Pharmaceutical Science
- Organic Chemistry
- Pharmacology (medical)