Suppressed formation of bone marrow adherent layers derived from acute myeloid leukemia patients after in vitro exposure to interleukin-4

Meir Wetzler; Razelle Kurzrock; Zeev Estrov; Steve Barone; Elihu Estey; Moshe Talpaz

doi:10.1016/S0145-2126(97)00005-2

Suppressed formation of bone marrow adherent layers derived from acute myeloid leukemia patients after in vitro exposure to interleukin-4

Meir Wetzler, Razelle Kurzrock, Zeev Estrov, Steve Barone, Elihu Estey, Moshe Talpaz

Graduate School of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Long-term bone marrow cultures from ten acute myeloid leukemia (AML) patients exposed to recombinant human (rhu) interleukin (IL) 4 from culture initiation failed to develop adherent layers at 5 weeks as compared to controls. The adherent layers from two of our patients expressed IL-1β transcripts constitutively, and all produced IL-6 and leukemia inhibitory factor (LIF) proteins. Our results demonstrate that rhulL-4 markedly inhibited AML-derived adherent layer formation in a time- and dose-dependent manner, and this effect was not mediated through enhanced apoptosis and did not correlate with IL-1β, IL-6 or LIF production.

Original language	English (US)
Pages (from-to)	519-527
Number of pages	9
Journal	Leukemia Research
Volume	21
Issue number	6
DOIs	https://doi.org/10.1016/S0145-2126(97)00005-2
State	Published - Jun 1997

Keywords

Acute myelogenous leukemia
Adherent layers
Cytokine
Interleukin

ASJC Scopus subject areas

Hematology
Oncology
Cancer Research

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10.1016/S0145-2126(97)00005-2

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title = "Suppressed formation of bone marrow adherent layers derived from acute myeloid leukemia patients after in vitro exposure to interleukin-4",

abstract = "Long-term bone marrow cultures from ten acute myeloid leukemia (AML) patients exposed to recombinant human (rhu) interleukin (IL) 4 from culture initiation failed to develop adherent layers at 5 weeks as compared to controls. The adherent layers from two of our patients expressed IL-1β transcripts constitutively, and all produced IL-6 and leukemia inhibitory factor (LIF) proteins. Our results demonstrate that rhulL-4 markedly inhibited AML-derived adherent layer formation in a time- and dose-dependent manner, and this effect was not mediated through enhanced apoptosis and did not correlate with IL-1β, IL-6 or LIF production.",

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T1 - Suppressed formation of bone marrow adherent layers derived from acute myeloid leukemia patients after in vitro exposure to interleukin-4

AU - Wetzler, Meir

AU - Kurzrock, Razelle

AU - Estrov, Zeev

AU - Barone, Steve

AU - Estey, Elihu

AU - Talpaz, Moshe

PY - 1997/6

Y1 - 1997/6

N2 - Long-term bone marrow cultures from ten acute myeloid leukemia (AML) patients exposed to recombinant human (rhu) interleukin (IL) 4 from culture initiation failed to develop adherent layers at 5 weeks as compared to controls. The adherent layers from two of our patients expressed IL-1β transcripts constitutively, and all produced IL-6 and leukemia inhibitory factor (LIF) proteins. Our results demonstrate that rhulL-4 markedly inhibited AML-derived adherent layer formation in a time- and dose-dependent manner, and this effect was not mediated through enhanced apoptosis and did not correlate with IL-1β, IL-6 or LIF production.

AB - Long-term bone marrow cultures from ten acute myeloid leukemia (AML) patients exposed to recombinant human (rhu) interleukin (IL) 4 from culture initiation failed to develop adherent layers at 5 weeks as compared to controls. The adherent layers from two of our patients expressed IL-1β transcripts constitutively, and all produced IL-6 and leukemia inhibitory factor (LIF) proteins. Our results demonstrate that rhulL-4 markedly inhibited AML-derived adherent layer formation in a time- and dose-dependent manner, and this effect was not mediated through enhanced apoptosis and did not correlate with IL-1β, IL-6 or LIF production.

KW - Acute myelogenous leukemia

KW - Adherent layers

KW - Cytokine

KW - Interleukin

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Suppressed formation of bone marrow adherent layers derived from acute myeloid leukemia patients after in vitro exposure to interleukin-4

Abstract

Keywords

ASJC Scopus subject areas

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