TY - JOUR
T1 - Survival of human diploid skin fibroblasts from normal individuals after x-irradiation
AU - Little, John B.
AU - Nove, John
AU - Strong, Louise C.
AU - Nichols, Warren W.
N1 - Funding Information:
Acknowledgements We thank William K . Dahlberg and Helen Vetrovs for expert technical assistance, and Dr Karl Kelsey for help with the statistical analyses . This research was supported by Research Grants CA-11751, CA-33624, CA-47542, and Center Grant ES-00002 from the US National Institutes of Health .
PY - 1988
Y1 - 1988
N2 - The cytotoxic effect of X-rays was measured by a colony formation assay in multiple experiments with fibroblast cell strains derived from 24 presumably normal individuals, received as 65 different coded and blinded samples. Each strain was received on two or more occasions at different times and bearing different codes. The means and standard deviations of the survival curve parameters for the 24 strains were: D0 = 123 ± 23; D10 = 273 ± 42 cGy. The D0 ranged from 89 to 175 and the D10 from 196 to 372 cGy. The degree of interexperimental variation, though generally minimal, differed considerably among cell strains. There was no systematic effect of passage level, cloning efficiency, serum lot, age or sex of the donor on X-ray survival. These results confirm that the intrinsic radiosensitivity varies significantly among skin fibroblasts isolated from clinically normal individuals, apparently owing to as yet unidentified genetic factors.
AB - The cytotoxic effect of X-rays was measured by a colony formation assay in multiple experiments with fibroblast cell strains derived from 24 presumably normal individuals, received as 65 different coded and blinded samples. Each strain was received on two or more occasions at different times and bearing different codes. The means and standard deviations of the survival curve parameters for the 24 strains were: D0 = 123 ± 23; D10 = 273 ± 42 cGy. The D0 ranged from 89 to 175 and the D10 from 196 to 372 cGy. The degree of interexperimental variation, though generally minimal, differed considerably among cell strains. There was no systematic effect of passage level, cloning efficiency, serum lot, age or sex of the donor on X-ray survival. These results confirm that the intrinsic radiosensitivity varies significantly among skin fibroblasts isolated from clinically normal individuals, apparently owing to as yet unidentified genetic factors.
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U2 - 10.1080/09553008814552311
DO - 10.1080/09553008814552311
M3 - Article
C2 - 2903888
AN - SCOPUS:0023744051
SN - 0955-3002
VL - 54
SP - 899
EP - 910
JO - International journal of radiation biology
JF - International journal of radiation biology
IS - 6
ER -