Synthesis of autophagosomal marker protein LC3-II under detergent-free conditions

Yi Chao Huang; Yi Ming Li; Yang Chen; Man Pan; Yi Tong Li; Li Yu; Qing Xiang Guo; Lei Liu

doi:10.1002/anie.201209523

Synthesis of autophagosomal marker protein LC3-II under detergent-free conditions

Yi Chao Huang, Yi Ming Li, Yang Chen, Man Pan, Yi Tong Li, Li Yu, Qing Xiang Guo, Lei Liu

Research output: Contribution to journal › Article › peer-review

97 Scopus citations

Abstract

Just add oil: A new detergent-free method was developed to synthesize lipidated proteins using a light-activatable solubilizing side chain (in dashed circle, see scheme) to assist the ligation of the lipopeptides. This method allows the efficient preparation of a phosphatidylethanolamine-conjugated autophagosomal marker protein (LC3-II) as well as labeled derivatives of LC3-II, which can be used to study autophagy regulation.

Original language	English (US)
Pages (from-to)	4858-4862
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	52
Issue number	18
DOIs	https://doi.org/10.1002/anie.201209523
State	Published - Apr 26 2013
Externally published	Yes

Keywords

LC3-II
autophagy
lipoproteins
protein expression
protein ligation

ASJC Scopus subject areas

Catalysis
General Chemistry

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10.1002/anie.201209523

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abstract = "Just add oil: A new detergent-free method was developed to synthesize lipidated proteins using a light-activatable solubilizing side chain (in dashed circle, see scheme) to assist the ligation of the lipopeptides. This method allows the efficient preparation of a phosphatidylethanolamine-conjugated autophagosomal marker protein (LC3-II) as well as labeled derivatives of LC3-II, which can be used to study autophagy regulation.",

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AU - Pan, Man

AU - Li, Yi Tong

AU - Yu, Li

AU - Guo, Qing Xiang

AU - Liu, Lei

PY - 2013/4/26

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AB - Just add oil: A new detergent-free method was developed to synthesize lipidated proteins using a light-activatable solubilizing side chain (in dashed circle, see scheme) to assist the ligation of the lipopeptides. This method allows the efficient preparation of a phosphatidylethanolamine-conjugated autophagosomal marker protein (LC3-II) as well as labeled derivatives of LC3-II, which can be used to study autophagy regulation.

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KW - autophagy

KW - lipoproteins

KW - protein expression

KW - protein ligation

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Synthesis of autophagosomal marker protein LC3-II under detergent-free conditions

Abstract

Keywords

ASJC Scopus subject areas

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