T-cell depletion by elutbiation. Comparison of bone marrow (BM) and mobilized peripheral blood (MoPB)

Normal human BM, and MoPB from breast cancer patients "mobilized" with G-CSF 10 μg/kg/day B.C. x4, were studied with counterflow centrifugal elutriation (CCE) (JE-5.0 elutriation rotor, 4 ml chamber, rotor speed at 2300 rpm and flow rate increases of 1 ml/min from 14-23 ml/min and R/0). T-cell concentrations and progenitor cells were assessed. Cumulative percentage of depletion at various now rules (ml/min) MoPB (#31 14 16 16 29 22 CD3⁺ 48±15 94±4 97±3 98±2 99±3 CD34⁺ 13±9 28±17 38±21 63±19 69±11 CFU-GM 1±2 17±11 14±30 67±31 89±18 HPP-CFC 0±0 8±13 28±16 62±33 96±7 BM (#4) CD3± 30±11 88±12 96±2 97±2 98±2 CD34⁺ 6±3 26±16 41±17 57±14 68±12 CFU-GM 1±2 23±17 49±12 69±7 80±6 HPP-CFC 4±4 46±24 83±7 97 ±3 100±0 Depletion of T-cells (CD3⁺), CD34⁺ cells, and CFU-GM was similar for BM and MoPB. CD3⁺ cells were depleted at lower flow-rates than CD34⁺ cells and CFU-GM. The early progenitor cell HPP-CFC appeared to be depleted at lower flow-rates from BM than from MoPB. These data suggest that T-cell depletion by CCE may remove fewer early progenitor cells from G-CSF mobilized peripheral blood than from bone marrow.