T-Cell immune surveillance in allogenic stem cell transplant recipients: Are whole blood-based assays ready to challenge ELISPOT?

Chris D. Lauruschkat; Lukas Page; Sonja Etter; Philipp Weis; Florian Gamon; Sabrina Kraus; Hermann Einsele; Sebastian Wurster; Juergen Loeffler

doi:10.1093/ofid/ofaa547

T-Cell immune surveillance in allogenic stem cell transplant recipients: Are whole blood-based assays ready to challenge ELISPOT?

Chris D. Lauruschkat, Lukas Page, Sonja Etter, Philipp Weis, Florian Gamon, Sabrina Kraus, Hermann Einsele, Sebastian Wurster, Juergen Loeffler

Infectious Diseases, Infection Control, and Employee Health

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

We compared the feasibility of 4 cytomegalovirus (CMV)- and Aspergillus-reactive T-cell immunoassay protocols in allogenic stem cell transplant recipients. While enzyme-linked immunospot performed best overall, logistically advantageous whole blood-based assays performed comparably in patients with less severe lymphocytopenia. CMV-induced interferon-gamma responses correlated strongly across all protocols and showed high concordance with serology.

Original language	English (US)
Article number	ofaa547
Journal	Open Forum Infectious Diseases
Volume	8
Issue number	1
DOIs	https://doi.org/10.1093/ofid/ofaa547
State	Published - Jan 1 2021

Keywords

Aspergillosis
Biomarker
Cytokines
Cytomegalovirus
ELISPOT
Flow cytometry
Immunoassay
T cells

ASJC Scopus subject areas

Oncology
Clinical Neurology

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abstract = "We compared the feasibility of 4 cytomegalovirus (CMV)- and Aspergillus-reactive T-cell immunoassay protocols in allogenic stem cell transplant recipients. While enzyme-linked immunospot performed best overall, logistically advantageous whole blood-based assays performed comparably in patients with less severe lymphocytopenia. CMV-induced interferon-gamma responses correlated strongly across all protocols and showed high concordance with serology.",

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AU - Page, Lukas

AU - Etter, Sonja

AU - Weis, Philipp

AU - Gamon, Florian

AU - Kraus, Sabrina

AU - Einsele, Hermann

AU - Wurster, Sebastian

AU - Loeffler, Juergen

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AB - We compared the feasibility of 4 cytomegalovirus (CMV)- and Aspergillus-reactive T-cell immunoassay protocols in allogenic stem cell transplant recipients. While enzyme-linked immunospot performed best overall, logistically advantageous whole blood-based assays performed comparably in patients with less severe lymphocytopenia. CMV-induced interferon-gamma responses correlated strongly across all protocols and showed high concordance with serology.

KW - Aspergillosis

KW - Biomarker

KW - Cytokines

KW - Cytomegalovirus

KW - ELISPOT

KW - Flow cytometry

KW - Immunoassay

KW - T cells

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T-Cell immune surveillance in allogenic stem cell transplant recipients: Are whole blood-based assays ready to challenge ELISPOT?

Abstract

Keywords

ASJC Scopus subject areas

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