Abstract
Introduction: Large granular lymphocyte leukemia (LGL) is a clonal lymphoproliferative disease of CD8+ T cells expressing the CD57 activation marker. It is, however, unknown whether the CD57+ population represents the LGL clone or not. We previously demonstrated that the clone can be found in both CD8+ CD57+ and CD8+ CD57- cells,indicating that the LGL clone also resides in the CD57- fraction. Materials and methods: Here, we quantified the extent of the clonal CD8 expansion in LGL using T-cell receptor Vβ (TCRBV)-specific monoclonal antibodies, and determined whether the CD4 population also contained skews. Furthermore, dominant TCRBV populations were assessed for clonal status using T-cell receptor-γ (TCRG) PCR on genomic DNA. Results: We show that the dominant TCRBV in LGL contains CD57+ and CD57- cells. Molecular analysis of CD8+ CD57+ and CD8+ CD57- subfractions of the dominant TCRBV by TCRG PCR demonstrates that indeed both fractions are clonal, and that the clone is absent from the dominant TCRBV-negative population. Furthermore, we show that CD57 overexpression is not restricted to the LGL clone, but a general phenomenon in CD8 cells of LGL patients. Conclusion: We therefore conclude that the primary characteristic of LGL is a clonal expansion of CD8 cells, with a concomitant upregulation of CD57 on this clone and uninvolved cells.
Original language | English (US) |
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Pages (from-to) | 18-25 |
Number of pages | 8 |
Journal | Hematology Journal |
Volume | 4 |
Issue number | 1 |
DOIs | |
State | Published - 2003 |
Externally published | Yes |
Keywords
- CD57
- Clonality
- LGL
- Memory/effector CD8 cells
ASJC Scopus subject areas
- Hematology