Targeted imaging of ovarian cancer cells using viral nanoparticles doped with indocyanine green

Yadir Guerrero; Baharak Bahmani; Bongsu Jung; Valentine Vullev; Vikas Kundra; Bahman Anvari

doi:10.1117/12.2006278

Targeted imaging of ovarian cancer cells using viral nanoparticles doped with indocyanine green

Yadir Guerrero, Baharak Bahmani, Bongsu Jung, Valentine Vullev, Vikas Kundra, Bahman Anvari

Image Guided Cancer Therapy Research Program

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Scopus citations

Abstract

Our group has constructed a new type of viral nanoparticles (VNPs) from genome-depleted plant infecting brome mosaic virus (BMV) that encapsulates the FDA-approved near infrared (NIR) indocyanine green (ICG)[1]. We refer to these VNPs as optical viral ghosts (OVGs) since the constructs lack the genomic content of wild-type BMV. One of our areas of interest is the application of OVGs for real-time intraoperative NIR fluorescence imaging of small peritoneal ovarian tumor nodules. We target human epidermal growth factor receptor-2 (HER-2) expression in ovarian cancer as a biomarker associated with ovarian cancer, since its over-expression is linked to the disease's progression to death. We functionalize the OVGs with anti-HER-2 monoclonal antibodies using reductive amination methods. We used fluorescence imaging to visualize the SKOV-3 cells (high HER-2 expression) after incubation with free ICG, OVGs, and functionalized OVGs. Our results suggest the possibility of using anti-HER2 conjugated OVGs in conjunction with cytoreductive surgery to detect small tumor nodules (<5cm) which currently are not excised during surgery.

Original language	English (US)
Title of host publication	Optical Methods for Tumor Treatment and Detection
Subtitle of host publication	Mechanisms and Techniques in Photodynamic Therapy XXII
DOIs	https://doi.org/10.1117/12.2006278
State	Published - 2013
Event	Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXII - San Francisco, CA, United States Duration: Feb 2 2013 → Feb 4 2013

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	8568
ISSN (Print)	1605-7422

Other

Other	Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXII
Country/Territory	United States
City	San Francisco, CA
Period	2/2/13 → 2/4/13

Keywords

Epithelial Growth Factor Receptor
Fluorescent Probe
Nanomedicine
Near-IR Imaging
Ovarian Cancer

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.2006278

Cite this

Guerrero, Y., Bahmani, B., Jung, B., Vullev, V., Kundra, V., & Anvari, B. (2013). Targeted imaging of ovarian cancer cells using viral nanoparticles doped with indocyanine green. In Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXII Article 856819 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8568). https://doi.org/10.1117/12.2006278

Targeted imaging of ovarian cancer cells using viral nanoparticles doped with indocyanine green. / Guerrero, Yadir; Bahmani, Baharak; Jung, Bongsu et al.
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXII. 2013. 856819 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8568).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Guerrero, Y, Bahmani, B, Jung, B, Vullev, V, Kundra, V & Anvari, B 2013, Targeted imaging of ovarian cancer cells using viral nanoparticles doped with indocyanine green. in Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXII., 856819, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 8568, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXII, San Francisco, CA, United States, 2/2/13. https://doi.org/10.1117/12.2006278

Guerrero Y, Bahmani B, Jung B, Vullev V, Kundra V, Anvari B. Targeted imaging of ovarian cancer cells using viral nanoparticles doped with indocyanine green. In Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXII. 2013. 856819. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2006278

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abstract = "Our group has constructed a new type of viral nanoparticles (VNPs) from genome-depleted plant infecting brome mosaic virus (BMV) that encapsulates the FDA-approved near infrared (NIR) indocyanine green (ICG)[1]. We refer to these VNPs as optical viral ghosts (OVGs) since the constructs lack the genomic content of wild-type BMV. One of our areas of interest is the application of OVGs for real-time intraoperative NIR fluorescence imaging of small peritoneal ovarian tumor nodules. We target human epidermal growth factor receptor-2 (HER-2) expression in ovarian cancer as a biomarker associated with ovarian cancer, since its over-expression is linked to the disease's progression to death. We functionalize the OVGs with anti-HER-2 monoclonal antibodies using reductive amination methods. We used fluorescence imaging to visualize the SKOV-3 cells (high HER-2 expression) after incubation with free ICG, OVGs, and functionalized OVGs. Our results suggest the possibility of using anti-HER2 conjugated OVGs in conjunction with cytoreductive surgery to detect small tumor nodules (<5cm) which currently are not excised during surgery.",

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author = "Yadir Guerrero and Baharak Bahmani and Bongsu Jung and Valentine Vullev and Vikas Kundra and Bahman Anvari",

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AB - Our group has constructed a new type of viral nanoparticles (VNPs) from genome-depleted plant infecting brome mosaic virus (BMV) that encapsulates the FDA-approved near infrared (NIR) indocyanine green (ICG)[1]. We refer to these VNPs as optical viral ghosts (OVGs) since the constructs lack the genomic content of wild-type BMV. One of our areas of interest is the application of OVGs for real-time intraoperative NIR fluorescence imaging of small peritoneal ovarian tumor nodules. We target human epidermal growth factor receptor-2 (HER-2) expression in ovarian cancer as a biomarker associated with ovarian cancer, since its over-expression is linked to the disease's progression to death. We functionalize the OVGs with anti-HER-2 monoclonal antibodies using reductive amination methods. We used fluorescence imaging to visualize the SKOV-3 cells (high HER-2 expression) after incubation with free ICG, OVGs, and functionalized OVGs. Our results suggest the possibility of using anti-HER2 conjugated OVGs in conjunction with cytoreductive surgery to detect small tumor nodules (<5cm) which currently are not excised during surgery.

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Targeted imaging of ovarian cancer cells using viral nanoparticles doped with indocyanine green

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