TY - JOUR
T1 - Targeted multifunctional gold-based nanoshells for magnetic resonance-guided laser ablation of head and neck cancer
AU - Melancon, Marites P.
AU - Lu, Wei
AU - Zhong, Meng
AU - Zhou, Min
AU - Liang, Gan
AU - Elliott, Andrew M.
AU - Hazle, John D.
AU - Myers, Jeffrey N.
AU - Li, Chun
AU - Jason Stafford, R.
N1 - Funding Information:
We thank Kristi M. Speights, ELS, for editing our manuscript. This work was supported in part by a grant from the National Institutes of Health ([C.L.] R01 CA119387 ), an ERG grant of SHSU (to G.L.), SPORE Head and Neck Career Development Award (to M.P.M., P50CA097007 ), and an Odyssey Fellowship (to M.P.M.). The Odyssey Fellowship is supported by the Odyssey Program and the Cockrell Foundation for Scientific Achievement at The University of Texas MD Anderson Cancer Center. This research was also supported in part by the National Institutes of Health through MD Anderson’s Cancer Center Support Grant ( CA016672 ) for the support of our small animal facility and small animal imaging facility.
PY - 2011/10
Y1 - 2011/10
N2 - Image-guided thermal ablation of tumors is becoming a more widely accepted minimally invasive alternative to surgery for patients who are not good surgical candidates, such as patients with advanced head and neck cancer. In this study, multifunctional superparamagnetic iron oxide coated with gold nanoshell (SPIO@Au NS) that have both optical and magnetic properties was conjugated with the targeting agent, C225 monoclonal antibody, against epidermal growth factor receptor (EGFR). C225-SPIO@Au NS have an average a diameter of 82 ± 4.4 nm, contain 142 ± 15 antibodies per nanoshell, have an absorption peak in the near infrared (∼800 nm), and have transverse relaxivity (r 2) of 193 and 353 mM -1 s -1 versus Feridex™ of 171 and 300 mM -1 s -1, using 1.5 T and 7 T MR scanners, respectively. Specific targeting of the synthesized C225-SPIO@Au NS was tested in vitro using A431 cells and oral cancer cells, FaDu, OSC19, and HN5, all of which overexpress EGFR. Selective binding was achieved using C225-SPIO@Au NS but not with the non-targeting PEG-SPIO@Au NS and blocking group (excess of C225 + C225-SPIO@Au NS). In vivo biodistribution on mice bearing A431 tumors also showed selective targeting of C225-SPIO@Au NS compared with the non-targeting and blocking groups. The selective photothermal ablation of the nanoshells shows that without laser treatment there were no cell death and among the groups that were treated with laser at a power of 36 W/cm 2 for 3 min, only the cells treated with C225-SPIO@Au NS had cell killing (p < 0.001). In summary, successful synthesis and characterization of targeted C225-SPIO@Au NS demonstrating both superparamagnetic and optical properties has been achieved. We have shown both in vitro and in vivo that these nanoshells are MR-active and can be selectively heated up for simultaneous imaging and photothermal ablation therapy.
AB - Image-guided thermal ablation of tumors is becoming a more widely accepted minimally invasive alternative to surgery for patients who are not good surgical candidates, such as patients with advanced head and neck cancer. In this study, multifunctional superparamagnetic iron oxide coated with gold nanoshell (SPIO@Au NS) that have both optical and magnetic properties was conjugated with the targeting agent, C225 monoclonal antibody, against epidermal growth factor receptor (EGFR). C225-SPIO@Au NS have an average a diameter of 82 ± 4.4 nm, contain 142 ± 15 antibodies per nanoshell, have an absorption peak in the near infrared (∼800 nm), and have transverse relaxivity (r 2) of 193 and 353 mM -1 s -1 versus Feridex™ of 171 and 300 mM -1 s -1, using 1.5 T and 7 T MR scanners, respectively. Specific targeting of the synthesized C225-SPIO@Au NS was tested in vitro using A431 cells and oral cancer cells, FaDu, OSC19, and HN5, all of which overexpress EGFR. Selective binding was achieved using C225-SPIO@Au NS but not with the non-targeting PEG-SPIO@Au NS and blocking group (excess of C225 + C225-SPIO@Au NS). In vivo biodistribution on mice bearing A431 tumors also showed selective targeting of C225-SPIO@Au NS compared with the non-targeting and blocking groups. The selective photothermal ablation of the nanoshells shows that without laser treatment there were no cell death and among the groups that were treated with laser at a power of 36 W/cm 2 for 3 min, only the cells treated with C225-SPIO@Au NS had cell killing (p < 0.001). In summary, successful synthesis and characterization of targeted C225-SPIO@Au NS demonstrating both superparamagnetic and optical properties has been achieved. We have shown both in vitro and in vivo that these nanoshells are MR-active and can be selectively heated up for simultaneous imaging and photothermal ablation therapy.
KW - Gold nanoshells
KW - Laser ablation
KW - Magnetic resonance imaging
KW - Theranostics
KW - Ultrasmall paramagnetic iron oxide (SPIO)
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U2 - 10.1016/j.biomaterials.2011.06.039
DO - 10.1016/j.biomaterials.2011.06.039
M3 - Article
C2 - 21745689
AN - SCOPUS:80051544063
SN - 0142-9612
VL - 32
SP - 7600
EP - 7608
JO - Biomaterials
JF - Biomaterials
IS - 30
ER -