Telomerase modulates Wnt signalling by association with target gene chromatin

Jae Il Park; Andrew S. Venteicher; Ji Yeon Hong; Jinkuk Choi; Sohee Jun; Marina Shkreli; Woody Chang; Zhaojing Meng; Peggie Cheung; Hong Ji; Margaret Mclaughlin; Timothy D. Veenstra; Roel Nusse; Pierre D. Mccrea; Steven E. Artandi

doi:10.1038/nature08137

Telomerase modulates Wnt signalling by association with target gene chromatin

Jae Il Park, Andrew S. Venteicher, Ji Yeon Hong, Jinkuk Choi, Sohee Jun, Marina Shkreli, Woody Chang, Zhaojing Meng, Peggie Cheung, Hong Ji, Margaret Mclaughlin, Timothy D. Veenstra, Roel Nusse, Pierre D. Mccrea, Steven E. Artandi

Research output: Contribution to journal › Review article › peer-review

559 Scopus citations

Abstract

Stem cells are controlled, in part, by genetic pathways frequently dysregulated during human tumorigenesis. Either stimulation of Wnt/Β-catenin signalling or overexpression of telomerase is sufficient to activate quiescent epidermal stem cells in vivo, although the mechanisms by which telomerase exerts these effects are not understood. Here we show that telomerase directly modulates Wnt/Β-catenin signalling by serving as a cofactor in a Β-catenin transcriptional complex. The telomerase protein component TERT (telomerase reverse transcriptase) interacts with BRG1 (also called SMARCA4), a SWI/SNF-related chromatin remodelling protein, and activates Wnt-dependent reporters in cultured cells and in vivo. TERT serves an essential role in formation of the anterior-posterior axis in Xenopus laevis embryos, and this defect in Wnt signalling manifests as homeotic transformations in the vertebrae of Tert^-/- mice. Chromatin immunoprecipitation of the endogenous TERT protein from mouse gastrointestinal tract shows that TERT physically occupies gene promoters of Wnt-dependent genes. These data reveal an unanticipated role for telomerase as a transcriptional modulator of the Wnt/Β-catenin signalling pathway.

Original language	English (US)
Pages (from-to)	66-72
Number of pages	7
Journal	Nature
Volume	460
Issue number	7251
DOIs	https://doi.org/10.1038/nature08137
State	Published - Jul 2 2009

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@article{87e1022349cf4b1483408b4763c0b075,

title = "Telomerase modulates Wnt signalling by association with target gene chromatin",

abstract = "Stem cells are controlled, in part, by genetic pathways frequently dysregulated during human tumorigenesis. Either stimulation of Wnt/Β-catenin signalling or overexpression of telomerase is sufficient to activate quiescent epidermal stem cells in vivo, although the mechanisms by which telomerase exerts these effects are not understood. Here we show that telomerase directly modulates Wnt/Β-catenin signalling by serving as a cofactor in a Β-catenin transcriptional complex. The telomerase protein component TERT (telomerase reverse transcriptase) interacts with BRG1 (also called SMARCA4), a SWI/SNF-related chromatin remodelling protein, and activates Wnt-dependent reporters in cultured cells and in vivo. TERT serves an essential role in formation of the anterior-posterior axis in Xenopus laevis embryos, and this defect in Wnt signalling manifests as homeotic transformations in the vertebrae of Tert-/- mice. Chromatin immunoprecipitation of the endogenous TERT protein from mouse gastrointestinal tract shows that TERT physically occupies gene promoters of Wnt-dependent genes. These data reveal an unanticipated role for telomerase as a transcriptional modulator of the Wnt/Β-catenin signalling pathway.",

author = "Park, {Jae Il} and Venteicher, {Andrew S.} and Hong, {Ji Yeon} and Jinkuk Choi and Sohee Jun and Marina Shkreli and Woody Chang and Zhaojing Meng and Peggie Cheung and Hong Ji and Margaret Mclaughlin and Veenstra, {Timothy D.} and Roel Nusse and Mccrea, {Pierre D.} and Artandi, {Steven E.}",

note = "Funding Information: Acknowledgements We thank P. Chu of the Stanford Comparative Medicine Histology Research Core Laboratory for technical assistance. We thank F. Ishikawa for Xenopus TERT plasmid, G. Crabtree for antibodies and plasmids, T. Jacks for ROSACreERT2 mice, and K. Park for constructive comments. J.-I.P. was supported by a Stanford Comprehensive Cancer Center Fellowship. This work was supported by NCI grants CA111691 and CA125453 and by a grant from the California Breast Cancer Research Program to S.E.A.",

year = "2009",

month = jul,

day = "2",

doi = "10.1038/nature08137",

language = "English (US)",

volume = "460",

pages = "66--72",

journal = "Nature",

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TY - JOUR

T1 - Telomerase modulates Wnt signalling by association with target gene chromatin

AU - Park, Jae Il

AU - Venteicher, Andrew S.

AU - Hong, Ji Yeon

AU - Choi, Jinkuk

AU - Jun, Sohee

AU - Shkreli, Marina

AU - Chang, Woody

AU - Meng, Zhaojing

AU - Cheung, Peggie

AU - Ji, Hong

AU - Mclaughlin, Margaret

AU - Veenstra, Timothy D.

AU - Nusse, Roel

AU - Mccrea, Pierre D.

AU - Artandi, Steven E.

N1 - Funding Information: Acknowledgements We thank P. Chu of the Stanford Comparative Medicine Histology Research Core Laboratory for technical assistance. We thank F. Ishikawa for Xenopus TERT plasmid, G. Crabtree for antibodies and plasmids, T. Jacks for ROSACreERT2 mice, and K. Park for constructive comments. J.-I.P. was supported by a Stanford Comprehensive Cancer Center Fellowship. This work was supported by NCI grants CA111691 and CA125453 and by a grant from the California Breast Cancer Research Program to S.E.A.

PY - 2009/7/2

Y1 - 2009/7/2

N2 - Stem cells are controlled, in part, by genetic pathways frequently dysregulated during human tumorigenesis. Either stimulation of Wnt/Β-catenin signalling or overexpression of telomerase is sufficient to activate quiescent epidermal stem cells in vivo, although the mechanisms by which telomerase exerts these effects are not understood. Here we show that telomerase directly modulates Wnt/Β-catenin signalling by serving as a cofactor in a Β-catenin transcriptional complex. The telomerase protein component TERT (telomerase reverse transcriptase) interacts with BRG1 (also called SMARCA4), a SWI/SNF-related chromatin remodelling protein, and activates Wnt-dependent reporters in cultured cells and in vivo. TERT serves an essential role in formation of the anterior-posterior axis in Xenopus laevis embryos, and this defect in Wnt signalling manifests as homeotic transformations in the vertebrae of Tert-/- mice. Chromatin immunoprecipitation of the endogenous TERT protein from mouse gastrointestinal tract shows that TERT physically occupies gene promoters of Wnt-dependent genes. These data reveal an unanticipated role for telomerase as a transcriptional modulator of the Wnt/Β-catenin signalling pathway.

AB - Stem cells are controlled, in part, by genetic pathways frequently dysregulated during human tumorigenesis. Either stimulation of Wnt/Β-catenin signalling or overexpression of telomerase is sufficient to activate quiescent epidermal stem cells in vivo, although the mechanisms by which telomerase exerts these effects are not understood. Here we show that telomerase directly modulates Wnt/Β-catenin signalling by serving as a cofactor in a Β-catenin transcriptional complex. The telomerase protein component TERT (telomerase reverse transcriptase) interacts with BRG1 (also called SMARCA4), a SWI/SNF-related chromatin remodelling protein, and activates Wnt-dependent reporters in cultured cells and in vivo. TERT serves an essential role in formation of the anterior-posterior axis in Xenopus laevis embryos, and this defect in Wnt signalling manifests as homeotic transformations in the vertebrae of Tert-/- mice. Chromatin immunoprecipitation of the endogenous TERT protein from mouse gastrointestinal tract shows that TERT physically occupies gene promoters of Wnt-dependent genes. These data reveal an unanticipated role for telomerase as a transcriptional modulator of the Wnt/Β-catenin signalling pathway.

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U2 - 10.1038/nature08137

DO - 10.1038/nature08137

M3 - Review article

C2 - 19571879

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SN - 0028-0836

VL - 460

SP - 66

EP - 72

JO - Nature

JF - Nature

IS - 7251

ER -

Telomerase modulates Wnt signalling by association with target gene chromatin

Abstract

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MD Anderson CCSG core facilities

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