Temporal profile of serum-induced S-phase entry and retinoblastoma protein phosphorylation in human skin fibroblasts

Farhad R. Danesh; Y. E. Minghao; Sasan Salmi; Michael Lapointe; Daniel Batlle

doi:10.1046/j.1523-1755.1999.00690.x

Temporal profile of serum-induced S-phase entry and retinoblastoma protein phosphorylation in human skin fibroblasts

Farhad R. Danesh, Y. E. Minghao, Sasan Salmi, Michael Lapointe, Daniel Batlle

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Cultured skin fibroblasts from type 1 diabetic patients with nephropathy have a hyperplastic growth phenotype in comparison with diabetics without nephropathy and controls. We studied the G1 phase cyclins in skin fibroblasts from control subjects to define the temporal profile of serum-induced pRB phosphorylation, cyclin D1 protein expression, and cyclin D1/CDK4(6) kinase activity as well as S-phase entry by FACS analysis. Our preliminary studies indicate that cultured skin fibroblasts from type 1 diabetic patients with nephropathy have an enhanced pRB phosphorylation, cyclin D1 protein expression, and cyclin D1/CDK4(6) kinase activity. This finding may become useful to identify patients at risk for the development of nephropathy.

Original language	English (US)
Pages (from-to)	1282-1285
Number of pages	4
Journal	Kidney International
Volume	56
Issue number	4
DOIs	https://doi.org/10.1046/j.1523-1755.1999.00690.x
State	Published - 1999
Externally published	Yes

Keywords

Cell growth
Glomerular mesangial cells
Hyperplastic growth phenotype
Nephropathy
Type 1 diabetes

ASJC Scopus subject areas

Nephrology

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10.1046/j.1523-1755.1999.00690.x

Cite this

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title = "Temporal profile of serum-induced S-phase entry and retinoblastoma protein phosphorylation in human skin fibroblasts",

abstract = "Cultured skin fibroblasts from type 1 diabetic patients with nephropathy have a hyperplastic growth phenotype in comparison with diabetics without nephropathy and controls. We studied the G1 phase cyclins in skin fibroblasts from control subjects to define the temporal profile of serum-induced pRB phosphorylation, cyclin D1 protein expression, and cyclin D1/CDK4(6) kinase activity as well as S-phase entry by FACS analysis. Our preliminary studies indicate that cultured skin fibroblasts from type 1 diabetic patients with nephropathy have an enhanced pRB phosphorylation, cyclin D1 protein expression, and cyclin D1/CDK4(6) kinase activity. This finding may become useful to identify patients at risk for the development of nephropathy.",

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AU - Danesh, Farhad R.

AU - Minghao, Y. E.

AU - Salmi, Sasan

AU - Lapointe, Michael

AU - Batlle, Daniel

PY - 1999

Y1 - 1999

N2 - Cultured skin fibroblasts from type 1 diabetic patients with nephropathy have a hyperplastic growth phenotype in comparison with diabetics without nephropathy and controls. We studied the G1 phase cyclins in skin fibroblasts from control subjects to define the temporal profile of serum-induced pRB phosphorylation, cyclin D1 protein expression, and cyclin D1/CDK4(6) kinase activity as well as S-phase entry by FACS analysis. Our preliminary studies indicate that cultured skin fibroblasts from type 1 diabetic patients with nephropathy have an enhanced pRB phosphorylation, cyclin D1 protein expression, and cyclin D1/CDK4(6) kinase activity. This finding may become useful to identify patients at risk for the development of nephropathy.

AB - Cultured skin fibroblasts from type 1 diabetic patients with nephropathy have a hyperplastic growth phenotype in comparison with diabetics without nephropathy and controls. We studied the G1 phase cyclins in skin fibroblasts from control subjects to define the temporal profile of serum-induced pRB phosphorylation, cyclin D1 protein expression, and cyclin D1/CDK4(6) kinase activity as well as S-phase entry by FACS analysis. Our preliminary studies indicate that cultured skin fibroblasts from type 1 diabetic patients with nephropathy have an enhanced pRB phosphorylation, cyclin D1 protein expression, and cyclin D1/CDK4(6) kinase activity. This finding may become useful to identify patients at risk for the development of nephropathy.

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KW - Hyperplastic growth phenotype

KW - Nephropathy

KW - Type 1 diabetes

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Temporal profile of serum-induced S-phase entry and retinoblastoma protein phosphorylation in human skin fibroblasts

Abstract

Keywords

ASJC Scopus subject areas

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