TY - JOUR
T1 - Testing serum batches for mouse embryonic stem cell culture
AU - Behringer, Richard
AU - Gertsenstein, Marina
AU - Nagy, Kristina Vintersten
AU - Nagy, Andras
N1 - Publisher Copyright:
© 2017 Cold Spring Harbor Laboratory Press.
PY - 2017/12
Y1 - 2017/12
N2 - The variability in embryonic stem (ES) cell culture is due primarily to serum. Serum is typically produced in large batches from many animals. However, samples may differ depending on the age and diet of the animals, the country of origin, and other factors creating lot-to-lot variations. Some vendors test FBS lots for compatibility with ES cell culture. Many laboratories prefer to test serum batches themselves to identify the lot giving optimal growth. In this protocol, small quantities of specific serum batches are obtained from different suppliers and tested for their ability to support ES cells in an undifferentiated state. A complete test includes the serum batches’ influence on plating efficiency, cell morphology, toxicity, and, if possible, their ability to support generation of chimeras.
AB - The variability in embryonic stem (ES) cell culture is due primarily to serum. Serum is typically produced in large batches from many animals. However, samples may differ depending on the age and diet of the animals, the country of origin, and other factors creating lot-to-lot variations. Some vendors test FBS lots for compatibility with ES cell culture. Many laboratories prefer to test serum batches themselves to identify the lot giving optimal growth. In this protocol, small quantities of specific serum batches are obtained from different suppliers and tested for their ability to support ES cells in an undifferentiated state. A complete test includes the serum batches’ influence on plating efficiency, cell morphology, toxicity, and, if possible, their ability to support generation of chimeras.
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U2 - 10.1101/pdb.prot092411
DO - 10.1101/pdb.prot092411
M3 - Article
C2 - 29196597
AN - SCOPUS:85037028574
SN - 1940-3402
VL - 2017
SP - 988
EP - 991
JO - Cold Spring Harbor protocols
JF - Cold Spring Harbor protocols
IS - 12
ER -