@article{3546f56131cd4af0bba7ecac1c4bb08e,
title = "Tetrahymena histone acetyltransferase A: A homolog to yeast Gcn5p linking histone acetylation to gene activation",
abstract = "We report the cloning of a transcription-associated histone acetyltransferase type A (HAT A). This Tetrahymena enzyme is strikingly homologous to the yeast protein Gcn5, a putative transcriptional adaptor, and we demonstrate that recombinant Gcn5p possesses HAT activity. Both the ciliate enzyme and Gcn5p contain potential active site residues found in other acetyltransferases and a highly conserved bromodomain. The presence of this domain in nuclear A-type HATs, but not in cytoplasmic B-type HATs, suggests a mechanism whereby HAT A is directed to chromatin to facilitate transcriptional activation. These findings shed light on the biochemical function of the evolutionarily conserved Gcn5p-Ada complex, directly linking histone acetylation to gene activation, and indicate that histone acetylation is a targeted phenomenon.",
author = "Brownell, {J. E.} and J. Zhou and T. Ranalli and R. Kobayashi and Edmondson, {D. G.} and Roth, {S. Y.} and Allis, {C. D.}",
note = "Funding Information: Correspondence should be addressed to C. D. A. We thank Mark Parthun, Dan Gottschling (University of Chicago), and Rolf Sternglanz (State University of New York at Stony Brook) for sharing the sequence of HAT1 and related data prior to its publication. We acknowledge Mark Brown for the large-scale growth of Tetrahymena and macronuclear isolations; Olga Yarygina for help in the cloning of GCN5 in the pRSET vector; Richard Cook and the protein core facility at the Baylor College of Medicine for synthesis of the p55 peptides; Lee Wong and the DNA sequencing facility at York University for help with DNA sequence analyses; Carol Greider and Bruce Stillman for establishing the collaboration with R. K. at the Cold Spring Harbor Laboratory; and Laura Kelley for help in the preparation of this manuscript. Insightful discussions with Martin Gorovsky, Laurie Stargell, Edwin Smith, and Jerry Workman are acknowledged. Portions of this study were carried out at the Department of Biology at Syracuse University. This work was funded by National Institutes of Health (NIH) grants to C. D. A. (GM53512), S. Y. R. (GM51189), and D. G. E. (F32CA65098) and by a National Cancer Institute grant to R. K. (CA13106). C. D. A. acknowledges the confidence the NIH have shown in our ability to approach this problem using Tetrahymena as a model. ",
year = "1996",
doi = "10.1016/S0092-8674(00)81063-6",
language = "English (US)",
volume = "84",
pages = "843--851",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "6",
}