Abstract
79 EST fragments which represents corresponding novel genes were obtained by sequencing and bioinformatics analysis of human fetal kidney cDNA library. Microarray was prepared by using these novel EST fragments by automatic spotting. Expression patters of 79 ESTs of novel genes from human fetal kidney were analyzed in fetal brain and fetal heart tissues of 20-weekage and 26-week-age fetus by performing of cDNA chip hybridization. This provides clues for studying exact functions of the novel genes. 8 genes were obtained which were expressed differentially in the fetal brain and heart of 20-week- and 26-week-age respectively. Then differentially expressed genes were identified by Northern analysis. The more exact function of the novel genes are under study.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 536 |
| Number of pages | 1 |
| Journal | Progress in Biochemistry and Biophysics |
| Volume | 28 |
| Issue number | 3 |
| State | Published - 2001 |
| Externally published | Yes |
Keywords
- cDNA chip
- Differential expression
- Expressed sequence tag (EST)
- Northern analysis
- Novel genes
ASJC Scopus subject areas
- Biophysics
- Biochemistry