The alteration of gene expression in rat liver during chemical carcinogenesis

Jen Fu Chiu; Dao Pei Huang; Anne L. Burkhardt; Gilbert Cote; Charles E. Schwartz

doi:10.1016/0003-9861(83)90528-3

The alteration of gene expression in rat liver during chemical carcinogenesis

Jen Fu Chiu, Dao Pei Huang, Anne L. Burkhardt, Gilbert Cote, Charles E. Schwartz

Endocrine Neoplasia & HD

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

The effect of the carcinogen 3′-methyl-4-dimethylaminoazobenzene (3′-MDAB) on the gene expression of rat liver was studied. Hybridization analysis with enriched neoplastic liver specific cDNA demonstrated a noticeable change in gene expression during liver carcinogenesis. The effect of 3′-MDAB on liver specific gene expression was also studied with albumin and α-fetoprotein (AFP) genes as models. Serum AFP levels increased dramatically during hepatocarcinogenesis. Amounts of AFP mRNA present in polysomal poly(A)mRNA were determined by translation and hybridization experiments. AFP mRNA increased in carcinogen-treated liver as the serum level of AFP increased. Increase of AFP mRNA in liver cytoplasmic RNAs can be detected as early as 18 days after feeding rats 3′-MDAB and reached peaks at 30-40 and 185 days of treatment. AFP serum level and AFP mRNA in liver cytosol decreased dramatically between these peaks, with a small amount of AFP mRNA and serum AFP detectable in rats treated with 3′-MDAB for 100 days. The increase in amount of AFP mRNA was due to an increase in transcription of the AFP gene in livers of rats treated with carcinogen. Only a slight change in serum albumin concentration and albumin mRNA in livers of rats treated with 3′-MDAB was noticed.

Original language	English (US)
Pages (from-to)	310-320
Number of pages	11
Journal	Archives of Biochemistry and Biophysics
Volume	222
Issue number	1
DOIs	https://doi.org/10.1016/0003-9861(83)90528-3
State	Published - Apr 1 1983

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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title = "The alteration of gene expression in rat liver during chemical carcinogenesis",

abstract = "The effect of the carcinogen 3′-methyl-4-dimethylaminoazobenzene (3′-MDAB) on the gene expression of rat liver was studied. Hybridization analysis with enriched neoplastic liver specific cDNA demonstrated a noticeable change in gene expression during liver carcinogenesis. The effect of 3′-MDAB on liver specific gene expression was also studied with albumin and α-fetoprotein (AFP) genes as models. Serum AFP levels increased dramatically during hepatocarcinogenesis. Amounts of AFP mRNA present in polysomal poly(A)mRNA were determined by translation and hybridization experiments. AFP mRNA increased in carcinogen-treated liver as the serum level of AFP increased. Increase of AFP mRNA in liver cytoplasmic RNAs can be detected as early as 18 days after feeding rats 3′-MDAB and reached peaks at 30-40 and 185 days of treatment. AFP serum level and AFP mRNA in liver cytosol decreased dramatically between these peaks, with a small amount of AFP mRNA and serum AFP detectable in rats treated with 3′-MDAB for 100 days. The increase in amount of AFP mRNA was due to an increase in transcription of the AFP gene in livers of rats treated with carcinogen. Only a slight change in serum albumin concentration and albumin mRNA in livers of rats treated with 3′-MDAB was noticed.",

author = "Chiu, {Jen Fu} and Huang, {Dao Pei} and Burkhardt, {Anne L.} and Gilbert Cote and Schwartz, {Charles E.}",

note = "Funding Information: This investigation was supported by USPHS Grant NC1 CA 25098. C. E. Schwartz was a predoctoral trainee of Cancer Biology Training, Grant No. T32 09286. The authors wish to thank Dr. Douglas P. Quick for providing enriched neoplastic liver cDNA. The authors are indebted to Drs. Davis L. Miller and James Bonner for their kindly providing rat AFP and albumin cloned cDNA pA5 and PRSA13. We thank Mr. Robert Shaw for preparation of this manuscript.",

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N1 - Funding Information: This investigation was supported by USPHS Grant NC1 CA 25098. C. E. Schwartz was a predoctoral trainee of Cancer Biology Training, Grant No. T32 09286. The authors wish to thank Dr. Douglas P. Quick for providing enriched neoplastic liver cDNA. The authors are indebted to Drs. Davis L. Miller and James Bonner for their kindly providing rat AFP and albumin cloned cDNA pA5 and PRSA13. We thank Mr. Robert Shaw for preparation of this manuscript.

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N2 - The effect of the carcinogen 3′-methyl-4-dimethylaminoazobenzene (3′-MDAB) on the gene expression of rat liver was studied. Hybridization analysis with enriched neoplastic liver specific cDNA demonstrated a noticeable change in gene expression during liver carcinogenesis. The effect of 3′-MDAB on liver specific gene expression was also studied with albumin and α-fetoprotein (AFP) genes as models. Serum AFP levels increased dramatically during hepatocarcinogenesis. Amounts of AFP mRNA present in polysomal poly(A)mRNA were determined by translation and hybridization experiments. AFP mRNA increased in carcinogen-treated liver as the serum level of AFP increased. Increase of AFP mRNA in liver cytoplasmic RNAs can be detected as early as 18 days after feeding rats 3′-MDAB and reached peaks at 30-40 and 185 days of treatment. AFP serum level and AFP mRNA in liver cytosol decreased dramatically between these peaks, with a small amount of AFP mRNA and serum AFP detectable in rats treated with 3′-MDAB for 100 days. The increase in amount of AFP mRNA was due to an increase in transcription of the AFP gene in livers of rats treated with carcinogen. Only a slight change in serum albumin concentration and albumin mRNA in livers of rats treated with 3′-MDAB was noticed.

AB - The effect of the carcinogen 3′-methyl-4-dimethylaminoazobenzene (3′-MDAB) on the gene expression of rat liver was studied. Hybridization analysis with enriched neoplastic liver specific cDNA demonstrated a noticeable change in gene expression during liver carcinogenesis. The effect of 3′-MDAB on liver specific gene expression was also studied with albumin and α-fetoprotein (AFP) genes as models. Serum AFP levels increased dramatically during hepatocarcinogenesis. Amounts of AFP mRNA present in polysomal poly(A)mRNA were determined by translation and hybridization experiments. AFP mRNA increased in carcinogen-treated liver as the serum level of AFP increased. Increase of AFP mRNA in liver cytoplasmic RNAs can be detected as early as 18 days after feeding rats 3′-MDAB and reached peaks at 30-40 and 185 days of treatment. AFP serum level and AFP mRNA in liver cytosol decreased dramatically between these peaks, with a small amount of AFP mRNA and serum AFP detectable in rats treated with 3′-MDAB for 100 days. The increase in amount of AFP mRNA was due to an increase in transcription of the AFP gene in livers of rats treated with carcinogen. Only a slight change in serum albumin concentration and albumin mRNA in livers of rats treated with 3′-MDAB was noticed.

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The alteration of gene expression in rat liver during chemical carcinogenesis

Abstract

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