TY - JOUR
T1 - The effect of bone morphogenetic protein-2 on osteosarcoma metastasis
AU - Gill, Jonathan
AU - Connolly, Patrick
AU - Roth, Michael
AU - Chung, So Hak
AU - Zhang, Wendong
AU - Piperdi, Sajida
AU - Hoang, Bang
AU - Yang, Rui
AU - Guzik, Hillary
AU - Morris, Jonathan
AU - Gorlick, Richard
AU - Geller, David S.
N1 - Funding Information:
This research was funded in part by Swim Across America, The Matthew Foster Foundation, Barbara Bates Center for The Study of the History of Nursing, and The Barbara Epstein Foundation. We would also like to thank the Pediatric Preclinical Testing Program for the use of their xenograft cell lines and Eugenie S. Kleinerman, MD who kindly provided the SaOS-LM7 cell line. We would also like to acknowledge Daewoong Pharmaceutical Co. Ltd., for graciously providing BMP-2.
Publisher Copyright:
© 2017 Gill et al.
PY - 2017/3
Y1 - 2017/3
N2 - Purpose Bone Morphogenetic Protein-2 (BMP-2) may offer the potential to enhance allograft-host osseous union in limb-salvage surgery following osteosarcoma resection. However, there is concern regarding the effect of locally applied BMP-2 on tumor recurrence and metastasis. The purpose of this project was to evaluate the effect of exogenous BMP-2 on osteosarcoma migration and invasion across a panel of tumor cell lines in vitro and to characterize the effect of BMP-2 on pulmonary osteosarcoma metastasis within a xenograft model. Experimental design The effect of BMP-2 on in vitro tumor growth and development was assessed across multiple standard and patient-derived xenograft osteosarcoma cell lines. Tumor migration capacity, invasion, and cell proliferation were characterized. In addition, the effect on metastasis was measured using a xenograft model following tail-vein injection. The effect of exogenous BMP-2 on the development of metastases was measured following both single and multiple BMP-2 administrations. Results There was no significant difference in migration capacity, invasion, or cell proliferation between the BMP-2 treated and the untreated osteosarcoma cell lines. There was no significant difference in pulmonary metastases between either the single-dose or multi-dose BMP-2 treated animals and the untreated control animals. Conclusions In the model systems tested, the addition of BMP-2 does not increase osteosarcoma proliferation, migration, invasion, or metastasis to the lungs.
AB - Purpose Bone Morphogenetic Protein-2 (BMP-2) may offer the potential to enhance allograft-host osseous union in limb-salvage surgery following osteosarcoma resection. However, there is concern regarding the effect of locally applied BMP-2 on tumor recurrence and metastasis. The purpose of this project was to evaluate the effect of exogenous BMP-2 on osteosarcoma migration and invasion across a panel of tumor cell lines in vitro and to characterize the effect of BMP-2 on pulmonary osteosarcoma metastasis within a xenograft model. Experimental design The effect of BMP-2 on in vitro tumor growth and development was assessed across multiple standard and patient-derived xenograft osteosarcoma cell lines. Tumor migration capacity, invasion, and cell proliferation were characterized. In addition, the effect on metastasis was measured using a xenograft model following tail-vein injection. The effect of exogenous BMP-2 on the development of metastases was measured following both single and multiple BMP-2 administrations. Results There was no significant difference in migration capacity, invasion, or cell proliferation between the BMP-2 treated and the untreated osteosarcoma cell lines. There was no significant difference in pulmonary metastases between either the single-dose or multi-dose BMP-2 treated animals and the untreated control animals. Conclusions In the model systems tested, the addition of BMP-2 does not increase osteosarcoma proliferation, migration, invasion, or metastasis to the lungs.
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U2 - 10.1371/journal.pone.0173322
DO - 10.1371/journal.pone.0173322
M3 - Article
C2 - 28264040
AN - SCOPUS:85014698776
SN - 1932-6203
VL - 12
JO - PloS one
JF - PloS one
IS - 3
M1 - e0173322
ER -