The effect of cyclin D1 overexpression in human head and neck cancer cells

Torahiko Nakashima; Yuichiro Kuratomi; Ryuji Yasumatsu; Muneyuki Masuda; Koji Koike; Toshiro Umezaki; Gary L. Clayman; Takashi Nakagawa; Shizuo Komune

doi:10.1007/s00405-004-0831-z

The effect of cyclin D1 overexpression in human head and neck cancer cells

Torahiko Nakashima, Yuichiro Kuratomi, Ryuji Yasumatsu, Muneyuki Masuda, Koji Koike, Toshiro Umezaki, Gary L. Clayman, Takashi Nakagawa, Shizuo Komune

Head & Neck Surgery

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Overexpression of cyclin D1 in head and neck cancer has been suggested to be a poor prognostic factor. To understand the role of cyclin D1 expression in head and neck cancer, we overexpressed cyclin D1 in TU182 (a cell line derived from pharyngeal cancer) using a retroviral vector. Stable transfectants were isolated by neomycin (G418) selection. Compared to the parental and control-vector transfected cells, the cyclin D1 transfected cells revealed a decrease of the G1/G0 population and resulted in continuous proliferation under low serum conditions. Proliferation assays revealed an increase in resistance to cisplatin in cyclin D1 overexpressing cells. These observation suggest that deregulation of cyclin D1 may reduce growth factor requirements and contribute to the resistance to some chemotherapeutic agents among head and neck cancer patients.

Original language	English (US)
Pages (from-to)	379-383
Number of pages	5
Journal	European Archives of Oto-Rhino-Laryngology
Volume	262
Issue number	5
DOIs	https://doi.org/10.1007/s00405-004-0831-z
State	Published - May 2005

Keywords

Cisplatin
Cyclin D1
Head and neck cancer

ASJC Scopus subject areas

Otorhinolaryngology

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10.1007/s00405-004-0831-z

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title = "The effect of cyclin D1 overexpression in human head and neck cancer cells",

abstract = "Overexpression of cyclin D1 in head and neck cancer has been suggested to be a poor prognostic factor. To understand the role of cyclin D1 expression in head and neck cancer, we overexpressed cyclin D1 in TU182 (a cell line derived from pharyngeal cancer) using a retroviral vector. Stable transfectants were isolated by neomycin (G418) selection. Compared to the parental and control-vector transfected cells, the cyclin D1 transfected cells revealed a decrease of the G1/G0 population and resulted in continuous proliferation under low serum conditions. Proliferation assays revealed an increase in resistance to cisplatin in cyclin D1 overexpressing cells. These observation suggest that deregulation of cyclin D1 may reduce growth factor requirements and contribute to the resistance to some chemotherapeutic agents among head and neck cancer patients.",

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AU - Nakashima, Torahiko

AU - Kuratomi, Yuichiro

AU - Yasumatsu, Ryuji

AU - Masuda, Muneyuki

AU - Koike, Koji

AU - Umezaki, Toshiro

AU - Clayman, Gary L.

AU - Nakagawa, Takashi

AU - Komune, Shizuo

PY - 2005/5

Y1 - 2005/5

N2 - Overexpression of cyclin D1 in head and neck cancer has been suggested to be a poor prognostic factor. To understand the role of cyclin D1 expression in head and neck cancer, we overexpressed cyclin D1 in TU182 (a cell line derived from pharyngeal cancer) using a retroviral vector. Stable transfectants were isolated by neomycin (G418) selection. Compared to the parental and control-vector transfected cells, the cyclin D1 transfected cells revealed a decrease of the G1/G0 population and resulted in continuous proliferation under low serum conditions. Proliferation assays revealed an increase in resistance to cisplatin in cyclin D1 overexpressing cells. These observation suggest that deregulation of cyclin D1 may reduce growth factor requirements and contribute to the resistance to some chemotherapeutic agents among head and neck cancer patients.

AB - Overexpression of cyclin D1 in head and neck cancer has been suggested to be a poor prognostic factor. To understand the role of cyclin D1 expression in head and neck cancer, we overexpressed cyclin D1 in TU182 (a cell line derived from pharyngeal cancer) using a retroviral vector. Stable transfectants were isolated by neomycin (G418) selection. Compared to the parental and control-vector transfected cells, the cyclin D1 transfected cells revealed a decrease of the G1/G0 population and resulted in continuous proliferation under low serum conditions. Proliferation assays revealed an increase in resistance to cisplatin in cyclin D1 overexpressing cells. These observation suggest that deregulation of cyclin D1 may reduce growth factor requirements and contribute to the resistance to some chemotherapeutic agents among head and neck cancer patients.

KW - Cisplatin

KW - Cyclin D1

KW - Head and neck cancer

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The effect of cyclin D1 overexpression in human head and neck cancer cells

Abstract

Keywords

ASJC Scopus subject areas

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