The effect of L-glutamic acid on the reception of cytokines by HL-60 cells

I. A. Kostanyan; R. I. Nurieva; E. V. Navolotskaya; M. V. Astapova; S. M. Dranitsyna; V. P. Zav'yalov; V. M. Lipkin

The effect of L-glutamic acid on the reception of cytokines by HL-60 cells

I. A. Kostanyan, R. I. Nurieva, E. V. Navolotskaya, M. V. Astapova, S. M. Dranitsyna, V. P. Zav'yalov, V. M. Lipkin

Research output: Contribution to journal › Article › peer-review

Abstract

L-Glutamic acid at a concentration of 0.1 μM was found to induce differentiation of the cell line of HL-60 promyelocytic leukemia into granulocytes or neutrophiles. The HL-60 cells have no specific glutamate-binding sites, but L-glutamic acid influences the reception of several cytokines by these cells. At a concentration of 0.1 μM, L-glutamic acid completely inhibits the high-affinity binding of ¹²⁵I-labeled human recombinant interleukin-1β (K_d = 0.32 nM) to the HL-60 cells, but does not affect their low-affinity binding (K_d = 13.3 nM) and does not change the total number of the IL-1β-binding sites. Preincubation of the HL-60 cells with 0.1 μM L-glutamic acid increases 2.5 times the number of receptors for ¹²⁵I-labeled human recombinant tumor necrosis factor β. These results suggest that L-glutamic acid plays an important role in the differentiation of the blood myeloid cells.

Original language	English (US)
Pages (from-to)	1-6
Number of pages	6
Journal	Russian Journal of Bioorganic Chemistry
Volume	24
Issue number	1
State	Published - 1998
Externally published	Yes

Keywords

Differentiation
Interleukin-1
L-glutamic acid
Receptors
Tumor necrosis factor

ASJC Scopus subject areas

Biochemistry
Organic Chemistry

Cite this

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title = "The effect of L-glutamic acid on the reception of cytokines by HL-60 cells",

abstract = "L-Glutamic acid at a concentration of 0.1 μM was found to induce differentiation of the cell line of HL-60 promyelocytic leukemia into granulocytes or neutrophiles. The HL-60 cells have no specific glutamate-binding sites, but L-glutamic acid influences the reception of several cytokines by these cells. At a concentration of 0.1 μM, L-glutamic acid completely inhibits the high-affinity binding of 125I-labeled human recombinant interleukin-1β (Kd = 0.32 nM) to the HL-60 cells, but does not affect their low-affinity binding (Kd = 13.3 nM) and does not change the total number of the IL-1β-binding sites. Preincubation of the HL-60 cells with 0.1 μM L-glutamic acid increases 2.5 times the number of receptors for 125I-labeled human recombinant tumor necrosis factor β. These results suggest that L-glutamic acid plays an important role in the differentiation of the blood myeloid cells.",

keywords = "Differentiation, Interleukin-1, L-glutamic acid, Receptors, Tumor necrosis factor",

author = "Kostanyan, {I. A.} and Nurieva, {R. I.} and Navolotskaya, {E. V.} and Astapova, {M. V.} and Dranitsyna, {S. M.} and Zav'yalov, {V. P.} and Lipkin, {V. M.}",

year = "1998",

language = "English (US)",

volume = "24",

pages = "1--6",

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AU - Kostanyan, I. A.

AU - Nurieva, R. I.

AU - Navolotskaya, E. V.

AU - Astapova, M. V.

AU - Dranitsyna, S. M.

AU - Zav'yalov, V. P.

AU - Lipkin, V. M.

PY - 1998

Y1 - 1998

N2 - L-Glutamic acid at a concentration of 0.1 μM was found to induce differentiation of the cell line of HL-60 promyelocytic leukemia into granulocytes or neutrophiles. The HL-60 cells have no specific glutamate-binding sites, but L-glutamic acid influences the reception of several cytokines by these cells. At a concentration of 0.1 μM, L-glutamic acid completely inhibits the high-affinity binding of 125I-labeled human recombinant interleukin-1β (Kd = 0.32 nM) to the HL-60 cells, but does not affect their low-affinity binding (Kd = 13.3 nM) and does not change the total number of the IL-1β-binding sites. Preincubation of the HL-60 cells with 0.1 μM L-glutamic acid increases 2.5 times the number of receptors for 125I-labeled human recombinant tumor necrosis factor β. These results suggest that L-glutamic acid plays an important role in the differentiation of the blood myeloid cells.

AB - L-Glutamic acid at a concentration of 0.1 μM was found to induce differentiation of the cell line of HL-60 promyelocytic leukemia into granulocytes or neutrophiles. The HL-60 cells have no specific glutamate-binding sites, but L-glutamic acid influences the reception of several cytokines by these cells. At a concentration of 0.1 μM, L-glutamic acid completely inhibits the high-affinity binding of 125I-labeled human recombinant interleukin-1β (Kd = 0.32 nM) to the HL-60 cells, but does not affect their low-affinity binding (Kd = 13.3 nM) and does not change the total number of the IL-1β-binding sites. Preincubation of the HL-60 cells with 0.1 μM L-glutamic acid increases 2.5 times the number of receptors for 125I-labeled human recombinant tumor necrosis factor β. These results suggest that L-glutamic acid plays an important role in the differentiation of the blood myeloid cells.

KW - Differentiation

KW - Interleukin-1

KW - L-glutamic acid

KW - Receptors

KW - Tumor necrosis factor

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The effect of L-glutamic acid on the reception of cytokines by HL-60 cells

Abstract

Keywords

ASJC Scopus subject areas

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