Abstract
Abstract - L-Glutamic acid at a concentration of 0.1 μM was found to induce differentiation of the cell line of HL-60 promyelocytic leukemia into granulocytes or neutrophiles. The HL-60 cells have no specific glutamate-binding sites, but L-glutamic acid influences the reception of several cytokines by these cells. At a concentration of 0.1 μM, L-glutamic acid completely inhibits the high-affinity binding of 125I-labeled human recombinant interleukin-1β (Kd = 0.32 nM) to the HL-60 cells, but does not affect their low-affinity binding (Kd = 13.3 nM) and does not change the total number of the IL-1β-binding sites. Preincubation of the HL-60 cells with 0.1 μM of L-glutamic acid increases 2.5 times the number of receptors for 125I-labeled human recombinant tumor necrosis factor β. These results suggest that L-glutamic acid plays an important role in the differentiation of the blood myeloid cells.
Original language | English (US) |
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Pages (from-to) | 8-9 |
Number of pages | 2 |
Journal | Bioorganicheskaya Khimiya |
Volume | 24 |
Issue number | 1 |
State | Published - 1998 |
Externally published | Yes |
Keywords
- Differentiation
- Interleukin-1
- L-glutamic acid
- Receptors
- Tumor necrosis factor
ASJC Scopus subject areas
- General Medicine