The effects of experimental conditions on the expression of in vitro-mediated tumor cytotoxicity mediated by murine macrophages

A. Raz; W. E. Fogler; I. J. Fidler

doi:10.1007/BF00199193

The effects of experimental conditions on the expression of in vitro-mediated tumor cytotoxicity mediated by murine macrophages

A. Raz, W. E. Fogler, I. J. Fidler

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67 Scopus citations

Abstract

The level of target destruction by tumoricidal mouse peritoneal macrophages was studied under various culture conditions. The degree of macrophage homogeneity in the monolayer was affected by the presence of fetal calf serum in the plating medium and the time allowed for cell adherence. Macrophage tumoricidal activity was not dependent on the ratio of macrophages to target cells, but on the densities of the two cell types in the culture vessel. The development of an in vitro microcytotoxicity assay for the evaluation of tumor cell lysis by activated macrophages revealed that serum concentration and the type of target cell used in the assay may affect the degree of tumoricidal response.

Original language	English (US)
Pages (from-to)	157-163
Number of pages	7
Journal	Cancer Immunology Immunotherapy
Volume	7
Issue number	3
DOIs	https://doi.org/10.1007/BF00199193
State	Published - Dec 1979
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Oncology
Cancer Research

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year = "1979",

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AU - Fidler, I. J.

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AB - The level of target destruction by tumoricidal mouse peritoneal macrophages was studied under various culture conditions. The degree of macrophage homogeneity in the monolayer was affected by the presence of fetal calf serum in the plating medium and the time allowed for cell adherence. Macrophage tumoricidal activity was not dependent on the ratio of macrophages to target cells, but on the densities of the two cell types in the culture vessel. The development of an in vitro microcytotoxicity assay for the evaluation of tumor cell lysis by activated macrophages revealed that serum concentration and the type of target cell used in the assay may affect the degree of tumoricidal response.

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The effects of experimental conditions on the expression of in vitro-mediated tumor cytotoxicity mediated by murine macrophages

Abstract

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