TY - JOUR
T1 - The estrogenic activity of DDT
T2 - The in vitro induction of an estrogen-inducible protein by o,p′-DDT
AU - Robison, Alice K.
AU - Mukku, Venkat R.
AU - Spalding, Diann M.
AU - Stancel, George M.
N1 - Funding Information:
’ Supported by NIH Grant HD-08615. Portions of this work were previously presented (ROBBON, A. K., MUKKU, V. R., IRELAND, J. S., AND STANCEL, G. M., (1982), Proc. Annu. Mtg. Sot. Toxicol. Abstr. 386. ’ Recipient of NIEHS Traineeship ES-07090. 3 o,p’-DDT, 2-(o-chlorophenyl)-2-(p-chlorophenyl)-1 , 1, I -trichloroethane; DATD, Nfl-diallyltartardiamide; Hepes, N-2-hydroxyethylpiperaxine-N’-2-ethanesulfonic acid, IP, induced protein, SDS, sodium dodecyl sulfate.
PY - 1984/12
Y1 - 1984/12
N2 - Induced protein is a commonly measured marker for estrogenic action. The induction of induced protein by o,p′-DDT was studied in an in vitro system. Nuclear levels of estrogen receptor translocated by o,p′-DDT correlated highly with induced protein induction, and the time course for induced protein induction was consistent with an estrogen receptor-mediated mechanism. While the maximum amount of induced protein produced by o,p′-DDT was less than after 17β-estradiol exposure, the induced protein formed by each compound was indistinguishable on nondenaturing and denaturing polyacrylamide gels. Also, it was shown that o,p′-DDT does not cause additional induction of induced protein over that seen with maximum levels of 17β-estradiol, further supporting the premise that these compounds share a common pathway in stimulating the synthesis of induced protein.
AB - Induced protein is a commonly measured marker for estrogenic action. The induction of induced protein by o,p′-DDT was studied in an in vitro system. Nuclear levels of estrogen receptor translocated by o,p′-DDT correlated highly with induced protein induction, and the time course for induced protein induction was consistent with an estrogen receptor-mediated mechanism. While the maximum amount of induced protein produced by o,p′-DDT was less than after 17β-estradiol exposure, the induced protein formed by each compound was indistinguishable on nondenaturing and denaturing polyacrylamide gels. Also, it was shown that o,p′-DDT does not cause additional induction of induced protein over that seen with maximum levels of 17β-estradiol, further supporting the premise that these compounds share a common pathway in stimulating the synthesis of induced protein.
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U2 - 10.1016/0041-008X(84)90358-2
DO - 10.1016/0041-008X(84)90358-2
M3 - Article
C2 - 6506078
AN - SCOPUS:0021742786
SN - 0041-008X
VL - 76
SP - 537
EP - 543
JO - Toxicology and Applied Pharmacology
JF - Toxicology and Applied Pharmacology
IS - 3
ER -