The evaluation of 186Re-labeled antibodies using N2S4 chelate in vitro and in vivo using tumor-bearing nude mice

A. Najafi; M. M. Alauddin; A. Sosa; G. Q. Ma; D. C.P. Chen; A. L. Epstein; M. E. Siegel

doi:10.1016/0883-2897(92)90009-N

The evaluation of ¹⁸⁶Re-labeled antibodies using N₂S₄ chelate in vitro and in vivo using tumor-bearing nude mice

A. Najafi, M. M. Alauddin, A. Sosa, G. Q. Ma, D. C.P. Chen, A. L. Epstein, M. E. Siegel

Cancer Systems Imaging

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Abstract

We have recently described a method for radiolabeling monoclonal antibodies, with metallic radionuclides using a new chelating agent N₂S₃. Using this chelate the monoclonal antibodies Lym-1 and B72.3 were labeled with ¹⁸⁶Re and their biological integrity was evaluated in vitro and in vivo. ¹⁸⁶Re-labeled antibodies using N₂S₄ methodology were found to be stable in human serum and retained their immunoreactivity. Intravenous administration of 0.5 mCi ¹⁸⁶Re-labeled antibodies resulted in partial or complete regression of tumor tissue in mice.

Original language	English (US)
Pages (from-to)	205-212
Number of pages	8
Journal	International Journal of Radiation Applications and Instrumentation.
Volume	19
Issue number	2
DOIs	https://doi.org/10.1016/0883-2897(92)90009-N
State	Published - Feb 1992

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General Medicine

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title = "The evaluation of 186Re-labeled antibodies using N2S4 chelate in vitro and in vivo using tumor-bearing nude mice",

abstract = "We have recently described a method for radiolabeling monoclonal antibodies, with metallic radionuclides using a new chelating agent N2S3. Using this chelate the monoclonal antibodies Lym-1 and B72.3 were labeled with 186Re and their biological integrity was evaluated in vitro and in vivo. 186Re-labeled antibodies using N2S4 methodology were found to be stable in human serum and retained their immunoreactivity. Intravenous administration of 0.5 mCi 186Re-labeled antibodies resulted in partial or complete regression of tumor tissue in mice.",

author = "A. Najafi and Alauddin, {M. M.} and A. Sosa and Ma, {G. Q.} and Chen, {D. C.P.} and Epstein, {A. L.} and Siegel, {M. E.}",

year = "1992",

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T1 - The evaluation of 186Re-labeled antibodies using N2S4 chelate in vitro and in vivo using tumor-bearing nude mice

AU - Najafi, A.

AU - Alauddin, M. M.

AU - Sosa, A.

AU - Ma, G. Q.

AU - Chen, D. C.P.

AU - Epstein, A. L.

AU - Siegel, M. E.

PY - 1992/2

Y1 - 1992/2

N2 - We have recently described a method for radiolabeling monoclonal antibodies, with metallic radionuclides using a new chelating agent N2S3. Using this chelate the monoclonal antibodies Lym-1 and B72.3 were labeled with 186Re and their biological integrity was evaluated in vitro and in vivo. 186Re-labeled antibodies using N2S4 methodology were found to be stable in human serum and retained their immunoreactivity. Intravenous administration of 0.5 mCi 186Re-labeled antibodies resulted in partial or complete regression of tumor tissue in mice.

AB - We have recently described a method for radiolabeling monoclonal antibodies, with metallic radionuclides using a new chelating agent N2S3. Using this chelate the monoclonal antibodies Lym-1 and B72.3 were labeled with 186Re and their biological integrity was evaluated in vitro and in vivo. 186Re-labeled antibodies using N2S4 methodology were found to be stable in human serum and retained their immunoreactivity. Intravenous administration of 0.5 mCi 186Re-labeled antibodies resulted in partial or complete regression of tumor tissue in mice.

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The evaluation of ¹⁸⁶Re-labeled antibodies using N₂S₄ chelate in vitro and in vivo using tumor-bearing nude mice

Abstract

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