TY - JOUR
T1 - The extended interface
T2 - Measuring non-local effects in biomolecular interactions
AU - Ladbury, John E.
AU - Williams, Mark A.
N1 - Funding Information:
We thank Jon Taylor and Radwan Fawaz for use of their unpublished data in Figure 3 . The authors’ work in this area is supported by a Wellcome Trust Senior Research Fellowship (JEL), BBSRC grant 31/C11098, and BBSRC, MRC and UCL Graduate School PhD Studentships.
PY - 2004/10
Y1 - 2004/10
N2 - Improvements in the sensitivity and availability of biophysical techniques for the detection of the formation of complexes in solution are revealing that the effects of binding are not restricted to the direct contacts between the biomolecules or even to a localised site. Rather, information about the binding event is transmitted throughout the biomolecules and the surrounding solution through changes in the hydrogen bonding, hydration and electrostatic field as the complex is formed. Calorimetric, volumetric and NMR methods are beginning to provide a quantitative view of the nature and thermodynamic consequences of this extended interface, and the resulting data pose a major challenge for computational models of binding.
AB - Improvements in the sensitivity and availability of biophysical techniques for the detection of the formation of complexes in solution are revealing that the effects of binding are not restricted to the direct contacts between the biomolecules or even to a localised site. Rather, information about the binding event is transmitted throughout the biomolecules and the surrounding solution through changes in the hydrogen bonding, hydration and electrostatic field as the complex is formed. Calorimetric, volumetric and NMR methods are beginning to provide a quantitative view of the nature and thermodynamic consequences of this extended interface, and the resulting data pose a major challenge for computational models of binding.
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U2 - 10.1016/j.sbi.2004.08.001
DO - 10.1016/j.sbi.2004.08.001
M3 - Review article
C2 - 15465316
AN - SCOPUS:4744338722
SN - 0959-440X
VL - 14
SP - 562
EP - 569
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
IS - 5
ER -