TY - JOUR
T1 - The identification and characterization of two phosphatidylinositol-4,5- bisphosphate 4-phosphatases
AU - Ungewickell, Alexander
AU - Hugge, Christopher
AU - Kisseleva, Marina
AU - Chang, Shao Chun
AU - Zou, Jun
AU - Feng, Yucheng
AU - Galyov, Edouard E.
AU - Wilson, Monita
AU - Majerus, Philip W.
PY - 2005/12/27
Y1 - 2005/12/27
N2 - Numerous inositol polyphosphate 5-phosphatases catalyze the degradation of phosphatidylinositol-4,5-bisphosphate (PtdIns-4,5-P2) to phosphatidylinositol-4-phosphate (PtdIns-4-P). An alternative pathway to degrade PtdIns-4,5-P2 is the hydrolysis of PtdIns-4,5-P2 by a 4-phosphatase, leading to the production of PtdIns-5-P. Whereas the bacterial IpgD enzyme is known to catalyze this reaction, no such mammalian enzyme has been found. We have identified and characterized two previously undescribed human enzymes, PtdIns-4,5-P2 4-phosphatase type I and type II, which catalyze the hydrolysis of PtdIns-4,5-P2 to phosphatidylinositol-5-phosphate (PtdIns-5-P). Both enzymes are ubiquitously expressed and localize to late endosomal lysosomal membranes in epithelial cells. Overexpression of either enzyme in HeLa cells increases EGF-receptor degradation upon EGF stimulation.
AB - Numerous inositol polyphosphate 5-phosphatases catalyze the degradation of phosphatidylinositol-4,5-bisphosphate (PtdIns-4,5-P2) to phosphatidylinositol-4-phosphate (PtdIns-4-P). An alternative pathway to degrade PtdIns-4,5-P2 is the hydrolysis of PtdIns-4,5-P2 by a 4-phosphatase, leading to the production of PtdIns-5-P. Whereas the bacterial IpgD enzyme is known to catalyze this reaction, no such mammalian enzyme has been found. We have identified and characterized two previously undescribed human enzymes, PtdIns-4,5-P2 4-phosphatase type I and type II, which catalyze the hydrolysis of PtdIns-4,5-P2 to phosphatidylinositol-5-phosphate (PtdIns-5-P). Both enzymes are ubiquitously expressed and localize to late endosomal lysosomal membranes in epithelial cells. Overexpression of either enzyme in HeLa cells increases EGF-receptor degradation upon EGF stimulation.
KW - Cell signaling
KW - Lysosome
KW - Phosphatidylinositol 5-phosphate
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U2 - 10.1073/pnas.0509740102
DO - 10.1073/pnas.0509740102
M3 - Article
C2 - 16365287
AN - SCOPUS:30044446496
SN - 0027-8424
VL - 102
SP - 18854
EP - 18859
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 52
ER -