TY - JOUR
T1 - The manufacture and assessment of tissue microarrays
T2 - Suggestions and criteria for analysis, with breast cancer as an example
AU - Pinder, Sarah E.
AU - Brown, John P.
AU - Gillett, Cheryl
AU - Purdie, Colin A.
AU - Speirs, Valerie
AU - Thompson, Alastair M.
AU - Shaaban, Abeer M.
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2013/3
Y1 - 2013/3
N2 - Tissue microarray (TMA) is an established and valuable tool, particularly in translational research and clinical trials, allowing resource-efficient use, and highthroughput profiling, of large numbers of tumours. Despite this, there is little evidence, or guidance, on the optimum manufacture, use and assessment of TMAs. Here we review some of the literature, using breast cancer as an example, to highlight good practice and pitfalls in the design and manufacture of TMAs. Issues, such as the size, number, spacing and layout of cores, as well as the assessment and reporting of studies using TMAs are addressed. We make some suggestions regarding these challenges, and propose a checklist of features that should be considered in order to stimulate debate and improve the quality of data produced by TMA analysis.
AB - Tissue microarray (TMA) is an established and valuable tool, particularly in translational research and clinical trials, allowing resource-efficient use, and highthroughput profiling, of large numbers of tumours. Despite this, there is little evidence, or guidance, on the optimum manufacture, use and assessment of TMAs. Here we review some of the literature, using breast cancer as an example, to highlight good practice and pitfalls in the design and manufacture of TMAs. Issues, such as the size, number, spacing and layout of cores, as well as the assessment and reporting of studies using TMAs are addressed. We make some suggestions regarding these challenges, and propose a checklist of features that should be considered in order to stimulate debate and improve the quality of data produced by TMA analysis.
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U2 - 10.1136/jclinpath-2012-201091
DO - 10.1136/jclinpath-2012-201091
M3 - Review article
C2 - 23087330
AN - SCOPUS:84874648124
SN - 0021-9746
VL - 66
SP - 169
EP - 177
JO - Journal of Clinical Pathology
JF - Journal of Clinical Pathology
IS - 3
ER -