Abstract
Inactivation of the dead-end (Dnd1) gene in the Ter mouse strain results in depletion of primordial germ cells (PGCs) so that mice become sterile. However, on the 129 mouse strain background, loss of Dnd1 also increases testicular germ cell tumor incidence in parallel to PGC depletion. We report that inactivation of Dnd1 also affects embryonic viability in the 129 strain. Mouse Dnd1 encodes two protein isoforms, DND1-isoform α (DND1-α) and DND1-isoform β (DND1-β). Using isoform-specific antibodies, we determined DND1-α is expressed in embryos and embryonic gonads whereas DND1-β expression is restricted to germ cells of the adult testis. Our data implicate DND1-α isoform to be necessary for germ cell viability and therefore its loss in Ter mice results in PGC depletion, germ cell tumor development and partial embryonic lethality in the 129 strain.
Original language | English (US) |
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Pages (from-to) | 194-199 |
Number of pages | 6 |
Journal | Biochemical and biophysical research communications |
Volume | 355 |
Issue number | 1 |
DOIs | |
State | Published - Mar 30 2007 |
Keywords
- Antibodies
- Dnd1
- Isoform
- Ter
- Testicular germ cell tumors
- dead-end
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
MD Anderson CCSG core facilities
- Genetically Engineered Mouse Facility