The MUC1 ectodomain: A novel and efficient target for gold nanoparticle clustering and vapor nanobubble generation

Brian P. Danysh; Pamela E. Constantinou; Ekaterina Y. Lukianova-Hleb; Dmitri O. Lapotko; Daniel D. Carson

doi:10.7150/thno.4494

The MUC1 ectodomain: A novel and efficient target for gold nanoparticle clustering and vapor nanobubble generation

Brian P. Danysh, Pamela E. Constantinou, Ekaterina Y. Lukianova-Hleb, Dmitri O. Lapotko, Daniel D. Carson

Endocrine Neoplasia & HD

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

MUC1 is a large, heavily glycosylated transmembrane glycoprotein that is proposed to create a protective microenvironment in many adenocarcinomas. Here we compare MUC1 and the well studied cell surface receptor target, EGFR, as gold nanoparticle (AuNP) targets and their subsequent vapor nanobubble generation efficacy in the human epithelial cell line, HES. Alt-hough EGFR and MUC1 were both highly expressed in these cells, TEM and confocal images revealed MUC1 as a superior target for nanoparticle intracellular accumulation and clustering. The MUC1-targeted AuNP intracellular clusters also generated significantly larger vapor nanobubbles. Our results demonstrate the promising opportunities MUC1 offers to improve the efficacy of targeted nanoparticle based approaches.

Original language	English (US)
Pages (from-to)	777-787
Number of pages	11
Journal	Theranostics
Volume	2
Issue number	8
DOIs	https://doi.org/10.7150/thno.4494
State	Published - 2012

Keywords

EGFR
MUC1
Nanoparticle Endo-cytosis
Nanoparticle clustering
Targeted gold nanoparticle
Vapor nanobubble

ASJC Scopus subject areas

Medicine (miscellaneous)
Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

Access to Document

10.7150/thno.4494

Cite this

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abstract = "MUC1 is a large, heavily glycosylated transmembrane glycoprotein that is proposed to create a protective microenvironment in many adenocarcinomas. Here we compare MUC1 and the well studied cell surface receptor target, EGFR, as gold nanoparticle (AuNP) targets and their subsequent vapor nanobubble generation efficacy in the human epithelial cell line, HES. Alt-hough EGFR and MUC1 were both highly expressed in these cells, TEM and confocal images revealed MUC1 as a superior target for nanoparticle intracellular accumulation and clustering. The MUC1-targeted AuNP intracellular clusters also generated significantly larger vapor nanobubbles. Our results demonstrate the promising opportunities MUC1 offers to improve the efficacy of targeted nanoparticle based approaches.",

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AU - Lukianova-Hleb, Ekaterina Y.

AU - Lapotko, Dmitri O.

AU - Carson, Daniel D.

PY - 2012

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AB - MUC1 is a large, heavily glycosylated transmembrane glycoprotein that is proposed to create a protective microenvironment in many adenocarcinomas. Here we compare MUC1 and the well studied cell surface receptor target, EGFR, as gold nanoparticle (AuNP) targets and their subsequent vapor nanobubble generation efficacy in the human epithelial cell line, HES. Alt-hough EGFR and MUC1 were both highly expressed in these cells, TEM and confocal images revealed MUC1 as a superior target for nanoparticle intracellular accumulation and clustering. The MUC1-targeted AuNP intracellular clusters also generated significantly larger vapor nanobubbles. Our results demonstrate the promising opportunities MUC1 offers to improve the efficacy of targeted nanoparticle based approaches.

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KW - Targeted gold nanoparticle

KW - Vapor nanobubble

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The MUC1 ectodomain: A novel and efficient target for gold nanoparticle clustering and vapor nanobubble generation

Abstract

Keywords

ASJC Scopus subject areas

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