The reactivity of anti-peptide antibodies is a function of the atomic mobility of sites in a protein

John A. Tainer; Elizabeth D. Getzoff; Hannah Alexander; Richard A. Houghten; Arthur J. Olson; Richard A. Lerner; Wayne A. Hendrickson

doi:10.1038/312127a0

The reactivity of anti-peptide antibodies is a function of the atomic mobility of sites in a protein

John A. Tainer, Elizabeth D. Getzoff, Hannah Alexander, Richard A. Houghten, Arthur J. Olson, Richard A. Lerner, Wayne A. Hendrickson

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358 Scopus citations

Abstract

To study the nature of antigenic recognition, antibodies have been prepared against a set of peptide sequences representing both highly mobile and well-ordered regions of myohaemerythrin, based on X-ray crystallographic temperature factors. Anti-peptide antibodies against highly mobile regions react strongly with the native protein; anti-peptide antibodies from well-ordered regions do not. Mobility is a major factor in the recognition of the native protein by anti-peptide antibodies; this may be of general significance in protein-protein interactions.

Original language	English (US)
Pages (from-to)	127-134
Number of pages	8
Journal	Nature
Volume	312
Issue number	5990
DOIs	https://doi.org/10.1038/312127a0
State	Published - 1984
Externally published	Yes

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abstract = "To study the nature of antigenic recognition, antibodies have been prepared against a set of peptide sequences representing both highly mobile and well-ordered regions of myohaemerythrin, based on X-ray crystallographic temperature factors. Anti-peptide antibodies against highly mobile regions react strongly with the native protein; anti-peptide antibodies from well-ordered regions do not. Mobility is a major factor in the recognition of the native protein by anti-peptide antibodies; this may be of general significance in protein-protein interactions.",

author = "Tainer, {John A.} and Getzoff, {Elizabeth D.} and Hannah Alexander and Houghten, {Richard A.} and Olson, {Arthur J.} and Lerner, {Richard A.} and Hendrickson, {Wayne A.}",

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doi = "10.1038/312127a0",

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AU - Tainer, John A.

AU - Getzoff, Elizabeth D.

AU - Alexander, Hannah

AU - Houghten, Richard A.

AU - Olson, Arthur J.

AU - Lerner, Richard A.

AU - Hendrickson, Wayne A.

PY - 1984

Y1 - 1984

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AB - To study the nature of antigenic recognition, antibodies have been prepared against a set of peptide sequences representing both highly mobile and well-ordered regions of myohaemerythrin, based on X-ray crystallographic temperature factors. Anti-peptide antibodies against highly mobile regions react strongly with the native protein; anti-peptide antibodies from well-ordered regions do not. Mobility is a major factor in the recognition of the native protein by anti-peptide antibodies; this may be of general significance in protein-protein interactions.

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JO - Nature

JF - Nature

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ER -

The reactivity of anti-peptide antibodies is a function of the atomic mobility of sites in a protein

Abstract

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