TY - JOUR
T1 - The Role of Dynamic Contrast-Enhanced MR in Evaluating Level III Collateral Circulation in a Rat Model of Acute Ischemic Stroke
AU - Chen, Hui
AU - Liu, Nan
AU - Li, Ying
AU - Wu, Bing
AU - Su, Zihua
AU - Zhu, Guangming
AU - Hu, Jun
N1 - Funding Information:
This article was supported by the National Natural Science Foundation of China (Grant Nos. 81371286 and 81501024), The National Natural Science Foundations of Beijing (Grant No. 7163226), and Clinical Innovation Foundation of Southwest Hospital (Grant No. SWH2013LC20).
Publisher Copyright:
© 2016, Springer Science+Business Media New York.
PY - 2017/5/1
Y1 - 2017/5/1
N2 - The study aimed to investigate the correlations between parameters of permeability magnetic resonance (MR) and the intensity of vasculature in the focal ischemic cortex of a rat model of middle cerebral artery occlusion. In the present study, sham operation or ischemic-reperfusion with tissue plasminogen activator (tPA) thrombolysis were performed in rats. Cranial MR scans and brain tissue samples were obtained from all animals. Brain slices were stained for immunohistological analyses with confocal fluorescence microscopy, and the intensities of microvessels and capillaries were calculated. Consistency between collateral circulation imaging classification and pathological vascular density was analyzed. We found that the Ktrans value and capillary intensity have the similar trends of declining and increasing, according to different time points, if compared with the sham operation group (P < 0.05). Permeability parameters have better correlations with capillary intensity, and Ktrans value had the best correlation at 4.5 h (kappa = 0.764, P < 0.001), while injection area under curve (IAUC) at 7 days (kappa = 0.725, P < 0.001). Susceptibility-weighted imaging (SWI) exhibited better correlation with microvessel intensity at both 3 days (kappa = 0.715, P < 0.001) and 7 days (kappa = 0.719, P < 0.001). In conclusion, the permeability parameters, such as IAUC and Ktrans map, can quantitatively assess capillary intensity, which may indicate angiogenesis categorized as level III collateral circulation after ischemic stroke.
AB - The study aimed to investigate the correlations between parameters of permeability magnetic resonance (MR) and the intensity of vasculature in the focal ischemic cortex of a rat model of middle cerebral artery occlusion. In the present study, sham operation or ischemic-reperfusion with tissue plasminogen activator (tPA) thrombolysis were performed in rats. Cranial MR scans and brain tissue samples were obtained from all animals. Brain slices were stained for immunohistological analyses with confocal fluorescence microscopy, and the intensities of microvessels and capillaries were calculated. Consistency between collateral circulation imaging classification and pathological vascular density was analyzed. We found that the Ktrans value and capillary intensity have the similar trends of declining and increasing, according to different time points, if compared with the sham operation group (P < 0.05). Permeability parameters have better correlations with capillary intensity, and Ktrans value had the best correlation at 4.5 h (kappa = 0.764, P < 0.001), while injection area under curve (IAUC) at 7 days (kappa = 0.725, P < 0.001). Susceptibility-weighted imaging (SWI) exhibited better correlation with microvessel intensity at both 3 days (kappa = 0.715, P < 0.001) and 7 days (kappa = 0.719, P < 0.001). In conclusion, the permeability parameters, such as IAUC and Ktrans map, can quantitatively assess capillary intensity, which may indicate angiogenesis categorized as level III collateral circulation after ischemic stroke.
KW - Collateral circulation
KW - Dynamic contrast-enhanced MR
KW - Permeability
KW - Stroke
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U2 - 10.1007/s12035-016-9844-z
DO - 10.1007/s12035-016-9844-z
M3 - Article
C2 - 27003821
AN - SCOPUS:84961780782
SN - 0893-7648
VL - 54
SP - 2731
EP - 2738
JO - Molecular Neurobiology
JF - Molecular Neurobiology
IS - 4
ER -