The serum immunoglobulin G glycosylation signature of gastric cancer

L. Renee Ruhaak; Donald A. Barkauskas; Javier Torres; Cara L. Cooke; Lauren D. Wu; Carol Stroble; Sureyya Ozcan; Cynthia C. Williams; Margarita Camorlinga; David M. Rocke; Carlito B. Lebrilla; Jay V. Solnick

doi:10.1016/j.euprot.2014.11.002

The serum immunoglobulin G glycosylation signature of gastric cancer

L. Renee Ruhaak, Donald A. Barkauskas, Javier Torres, Cara L. Cooke, Lauren D. Wu, Carol Stroble, Sureyya Ozcan, Cynthia C. Williams, Margarita Camorlinga, David M. Rocke, Carlito B. Lebrilla, Jay V. Solnick

Translational Molecular Pathology

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

Biomarkers may facilitate detection of gastric cancer at an earlier stage and reduce mortality. Here we sought to determine if the glycosylation profile of serum immunoglobulin G (IgG) could distinguish patients with non-atrophic gastritis (NAG), duodenal ulcer (DU) and gastric cancer (GC). Serum IgG was released and analyzed using nano-LC-TOF mass spectrometry. Statistically significant false discovery rate (FDR)-adjusted p-values were observed for 18 glycans, eight that differed significantly between NAG and GC, three that distinguished NAG from DU, and eight that differed between DU and GC. The IgG glycosylation signature may be useful as a predictive marker for gastric cancer.

Original language	English (US)
Pages (from-to)	1-9
Number of pages	9
Journal	EuPA Open Proteomics
Volume	6
DOIs	https://doi.org/10.1016/j.euprot.2014.11.002
State	Published - Mar 1 2015

Keywords

Duodenal ulcer
Gastric cancer
Gastritis
N-glycan
Nano-LC-MS
Serum

ASJC Scopus subject areas

Biochemistry

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10.1016/j.euprot.2014.11.002

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title = "The serum immunoglobulin G glycosylation signature of gastric cancer",

abstract = "Biomarkers may facilitate detection of gastric cancer at an earlier stage and reduce mortality. Here we sought to determine if the glycosylation profile of serum immunoglobulin G (IgG) could distinguish patients with non-atrophic gastritis (NAG), duodenal ulcer (DU) and gastric cancer (GC). Serum IgG was released and analyzed using nano-LC-TOF mass spectrometry. Statistically significant false discovery rate (FDR)-adjusted p-values were observed for 18 glycans, eight that differed significantly between NAG and GC, three that distinguished NAG from DU, and eight that differed between DU and GC. The IgG glycosylation signature may be useful as a predictive marker for gastric cancer.",

keywords = "Duodenal ulcer, Gastric cancer, Gastritis, N-glycan, Nano-LC-MS, Serum",

author = "Ruhaak, {L. Renee} and Barkauskas, {Donald A.} and Javier Torres and Cooke, {Cara L.} and Wu, {Lauren D.} and Carol Stroble and Sureyya Ozcan and Williams, {Cynthia C.} and Margarita Camorlinga and Rocke, {David M.} and Lebrilla, {Carlito B.} and Solnick, {Jay V.}",

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AU - Ruhaak, L. Renee

AU - Barkauskas, Donald A.

AU - Torres, Javier

AU - Cooke, Cara L.

AU - Wu, Lauren D.

AU - Stroble, Carol

AU - Ozcan, Sureyya

AU - Williams, Cynthia C.

AU - Camorlinga, Margarita

AU - Rocke, David M.

AU - Lebrilla, Carlito B.

AU - Solnick, Jay V.

PY - 2015/3/1

Y1 - 2015/3/1

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AB - Biomarkers may facilitate detection of gastric cancer at an earlier stage and reduce mortality. Here we sought to determine if the glycosylation profile of serum immunoglobulin G (IgG) could distinguish patients with non-atrophic gastritis (NAG), duodenal ulcer (DU) and gastric cancer (GC). Serum IgG was released and analyzed using nano-LC-TOF mass spectrometry. Statistically significant false discovery rate (FDR)-adjusted p-values were observed for 18 glycans, eight that differed significantly between NAG and GC, three that distinguished NAG from DU, and eight that differed between DU and GC. The IgG glycosylation signature may be useful as a predictive marker for gastric cancer.

KW - Duodenal ulcer

KW - Gastric cancer

KW - Gastritis

KW - N-glycan

KW - Nano-LC-MS

KW - Serum

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The serum immunoglobulin G glycosylation signature of gastric cancer

Abstract

Keywords

ASJC Scopus subject areas

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