The Set2 methyltransferase associates with Ssn6 yet Tup1-Ssn6 repression is independent of histone methylation

Tamara Tripic; Diane G. Edmondson; Judith K. Davie; Brian D. Strahl; Sharon Y.R. Dent

doi:10.1016/j.bbrc.2005.11.103

The Set2 methyltransferase associates with Ssn6 yet Tup1-Ssn6 repression is independent of histone methylation

Tamara Tripic, Diane G. Edmondson, Judith K. Davie, Brian D. Strahl, Sharon Y.R. Dent

Epigenetics & Molecular Carcinogenesis

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The Tup1-Ssn6 corepressor regulates the expression of diverse classes of genes in Saccharomyces cerevisiae. Chromatin is an important component of Tup1-Ssn6-mediated repression. Tup1 binds to underacetylated tails of histones H3 and H4, and requires multiple histone deacetylases for the repression. Here we examine if histone methylation, in addition to histone deacetylation, plays a role in Tup1-Ssn6 repression. We found that like other genes, Tup1-Ssn6 target genes exhibit increased levels of histone H3 lysine 4 trimethylation upon activation. However, deletion of individual or multiple histone methyltransferases and other SET-domain containing genes has no apparent effect on Tup1-Ssn6-mediated repression of a number of well-defined targets. Interestingly, we discovered that Ssn6 interacts with Set2. Although deletion of SET2 does not affect Tup1-Ssn6 repression of a number of target genes, Ssn6 may utilize Set2 in specific contexts to regulate gene repression.

Original language	English (US)
Pages (from-to)	905-914
Number of pages	10
Journal	Biochemical and biophysical research communications
Volume	339
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2005.11.103
State	Published - Jan 20 2006

Keywords

Chromatin
Histone
Methyltransferase
Repression
Yeast

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2005.11.103

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title = "The Set2 methyltransferase associates with Ssn6 yet Tup1-Ssn6 repression is independent of histone methylation",

abstract = "The Tup1-Ssn6 corepressor regulates the expression of diverse classes of genes in Saccharomyces cerevisiae. Chromatin is an important component of Tup1-Ssn6-mediated repression. Tup1 binds to underacetylated tails of histones H3 and H4, and requires multiple histone deacetylases for the repression. Here we examine if histone methylation, in addition to histone deacetylation, plays a role in Tup1-Ssn6 repression. We found that like other genes, Tup1-Ssn6 target genes exhibit increased levels of histone H3 lysine 4 trimethylation upon activation. However, deletion of individual or multiple histone methyltransferases and other SET-domain containing genes has no apparent effect on Tup1-Ssn6-mediated repression of a number of well-defined targets. Interestingly, we discovered that Ssn6 interacts with Set2. Although deletion of SET2 does not affect Tup1-Ssn6 repression of a number of target genes, Ssn6 may utilize Set2 in specific contexts to regulate gene repression.",

keywords = "Chromatin, Histone, Methyltransferase, Repression, Yeast",

author = "Tamara Tripic and Edmondson, {Diane G.} and Davie, {Judith K.} and Strahl, {Brian D.} and Dent, {Sharon Y.R.}",

note = "Funding Information: This work was supported by National Institutes of Health Grant GM51189 (to S.Y.R.D.). T.T. was supported by Robert A. Welch Foundation Grant 1371. We thank M.M. Coombes for help with RNA preparations.",

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AU - Tripic, Tamara

AU - Edmondson, Diane G.

AU - Davie, Judith K.

AU - Strahl, Brian D.

AU - Dent, Sharon Y.R.

N1 - Funding Information: This work was supported by National Institutes of Health Grant GM51189 (to S.Y.R.D.). T.T. was supported by Robert A. Welch Foundation Grant 1371. We thank M.M. Coombes for help with RNA preparations.

PY - 2006/1/20

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N2 - The Tup1-Ssn6 corepressor regulates the expression of diverse classes of genes in Saccharomyces cerevisiae. Chromatin is an important component of Tup1-Ssn6-mediated repression. Tup1 binds to underacetylated tails of histones H3 and H4, and requires multiple histone deacetylases for the repression. Here we examine if histone methylation, in addition to histone deacetylation, plays a role in Tup1-Ssn6 repression. We found that like other genes, Tup1-Ssn6 target genes exhibit increased levels of histone H3 lysine 4 trimethylation upon activation. However, deletion of individual or multiple histone methyltransferases and other SET-domain containing genes has no apparent effect on Tup1-Ssn6-mediated repression of a number of well-defined targets. Interestingly, we discovered that Ssn6 interacts with Set2. Although deletion of SET2 does not affect Tup1-Ssn6 repression of a number of target genes, Ssn6 may utilize Set2 in specific contexts to regulate gene repression.

AB - The Tup1-Ssn6 corepressor regulates the expression of diverse classes of genes in Saccharomyces cerevisiae. Chromatin is an important component of Tup1-Ssn6-mediated repression. Tup1 binds to underacetylated tails of histones H3 and H4, and requires multiple histone deacetylases for the repression. Here we examine if histone methylation, in addition to histone deacetylation, plays a role in Tup1-Ssn6 repression. We found that like other genes, Tup1-Ssn6 target genes exhibit increased levels of histone H3 lysine 4 trimethylation upon activation. However, deletion of individual or multiple histone methyltransferases and other SET-domain containing genes has no apparent effect on Tup1-Ssn6-mediated repression of a number of well-defined targets. Interestingly, we discovered that Ssn6 interacts with Set2. Although deletion of SET2 does not affect Tup1-Ssn6 repression of a number of target genes, Ssn6 may utilize Set2 in specific contexts to regulate gene repression.

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The Set2 methyltransferase associates with Ssn6 yet Tup1-Ssn6 repression is independent of histone methylation

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Keywords

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