The specific binding of transferrin to the murine fibroblast cell lines AKR-2B and its malignant counterpart AKR-MCA may be related to the transformed state

Douglas Boyd; Stan Bialoski; Michael G. Brattain

doi:10.1016/0304-3835(87)90019-X

The specific binding of transferrin to the murine fibroblast cell lines AKR-2B and its malignant counterpart AKR-MCA may be related to the transformed state

Douglas Boyd, Stan Bialoski, Michael G. Brattain

Cancer Biology

Research output: Contribution to journal › Article › peer-review

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Abstract

The specific binding of radiolabelled transferrin to AKR-2B and AKR-MCA murine fibroblasts was studied. The binding of radioligand to both cell lines was specific, being displaced by excess of unlabelled transferrin but not myoglobin, or lactoperoxidase. Under equilibrium conditions the transformed line AKR-MCA bound significantly more radioactive transferrin (22.5 ± 3.5 fmol/μg DNA) than the parental line AKR-2B (14.5 ± 1.5 fmol/μg DNA). The differences in the amount of ligand bound was due to altered receptor numbers. Treatment of AKR-MCA and AKR-2B cells with DMF eliminated the difference in transferrin binding capacities. The maximum decrease in specific ligand binding to AKR-MCA cells brought about by the polar solvent was observed after 48 h. These data suggest an association between transferrin binding and the transformed state of AKR-2B fibroblasts.

Original language	English (US)
Pages (from-to)	263-271
Number of pages	9
Journal	Cancer Letters
Volume	36
Issue number	3
DOIs	https://doi.org/10.1016/0304-3835(87)90019-X
State	Published - Sep 1987

ASJC Scopus subject areas

Oncology
Cancer Research

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@article{65bb665024bb457ebb42a4ff652c0518,

title = "The specific binding of transferrin to the murine fibroblast cell lines AKR-2B and its malignant counterpart AKR-MCA may be related to the transformed state",

abstract = "The specific binding of radiolabelled transferrin to AKR-2B and AKR-MCA murine fibroblasts was studied. The binding of radioligand to both cell lines was specific, being displaced by excess of unlabelled transferrin but not myoglobin, or lactoperoxidase. Under equilibrium conditions the transformed line AKR-MCA bound significantly more radioactive transferrin (22.5 ± 3.5 fmol/μg DNA) than the parental line AKR-2B (14.5 ± 1.5 fmol/μg DNA). The differences in the amount of ligand bound was due to altered receptor numbers. Treatment of AKR-MCA and AKR-2B cells with DMF eliminated the difference in transferrin binding capacities. The maximum decrease in specific ligand binding to AKR-MCA cells brought about by the polar solvent was observed after 48 h. These data suggest an association between transferrin binding and the transformed state of AKR-2B fibroblasts.",

author = "Douglas Boyd and Stan Bialoski and Brattain, {Michael G.}",

note = "Funding Information: *This work was supported by NIH grants CA34432 and CA38173. Abbreviations: DMF, N,WdimethyIfommui~ SDS, sodium dodecyl suifa* DMSO, dimeW- sulphoside; BSA, bovine serum albumin; Trf, trausferriq PBS, phosphate buffered saline (NaCI 0.15 M, NaH${\textquoteleft}G, 0.666 M, NsHF{\textquoteright}C, 0.605 M). Address correspondence to: Michael G. Brattain.",

year = "1987",

month = sep,

doi = "10.1016/0304-3835(87)90019-X",

language = "English (US)",

volume = "36",

pages = "263--271",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "3",

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T1 - The specific binding of transferrin to the murine fibroblast cell lines AKR-2B and its malignant counterpart AKR-MCA may be related to the transformed state

AU - Boyd, Douglas

AU - Bialoski, Stan

AU - Brattain, Michael G.

N1 - Funding Information: *This work was supported by NIH grants CA34432 and CA38173. Abbreviations: DMF, N,WdimethyIfommui~ SDS, sodium dodecyl suifa* DMSO, dimeW- sulphoside; BSA, bovine serum albumin; Trf, trausferriq PBS, phosphate buffered saline (NaCI 0.15 M, NaH$‘G, 0.666 M, NsHF’C, 0.605 M). Address correspondence to: Michael G. Brattain.

PY - 1987/9

Y1 - 1987/9

N2 - The specific binding of radiolabelled transferrin to AKR-2B and AKR-MCA murine fibroblasts was studied. The binding of radioligand to both cell lines was specific, being displaced by excess of unlabelled transferrin but not myoglobin, or lactoperoxidase. Under equilibrium conditions the transformed line AKR-MCA bound significantly more radioactive transferrin (22.5 ± 3.5 fmol/μg DNA) than the parental line AKR-2B (14.5 ± 1.5 fmol/μg DNA). The differences in the amount of ligand bound was due to altered receptor numbers. Treatment of AKR-MCA and AKR-2B cells with DMF eliminated the difference in transferrin binding capacities. The maximum decrease in specific ligand binding to AKR-MCA cells brought about by the polar solvent was observed after 48 h. These data suggest an association between transferrin binding and the transformed state of AKR-2B fibroblasts.

AB - The specific binding of radiolabelled transferrin to AKR-2B and AKR-MCA murine fibroblasts was studied. The binding of radioligand to both cell lines was specific, being displaced by excess of unlabelled transferrin but not myoglobin, or lactoperoxidase. Under equilibrium conditions the transformed line AKR-MCA bound significantly more radioactive transferrin (22.5 ± 3.5 fmol/μg DNA) than the parental line AKR-2B (14.5 ± 1.5 fmol/μg DNA). The differences in the amount of ligand bound was due to altered receptor numbers. Treatment of AKR-MCA and AKR-2B cells with DMF eliminated the difference in transferrin binding capacities. The maximum decrease in specific ligand binding to AKR-MCA cells brought about by the polar solvent was observed after 48 h. These data suggest an association between transferrin binding and the transformed state of AKR-2B fibroblasts.

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The specific binding of transferrin to the murine fibroblast cell lines AKR-2B and its malignant counterpart AKR-MCA may be related to the transformed state

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