TY - JOUR
T1 - The Src family kinase Fyn mediates signals induced by TCR antagonists
AU - Tang, Qizhi
AU - Subudhi, Sumit K.
AU - Henriksen, Kammi J.
AU - Long, Catherine G.
AU - Vives, Franklin
AU - Bluestone, Jeffrey A.
PY - 2002/5/1
Y1 - 2002/5/1
N2 - FcR nonbinding anti-CD3ε mAbs elicit partial TCR signaling that leads to T cell unresponsiveness and tolerance in vivo. In this study, the membrane-proximal events that promote T cell inactivation by FcR nonbinding anti-CD3 mAbs were examined. In the context of FcR nonbinding anti-CD3, TCR complexes did not aggregate and failed to translocate into glycolipid-enriched membrane microdomains. Furthermore, FcR nonbinding anti-CD3 mAbs induced tyrosine phosphorylation of the Fyn substrate Cbl, but not the ZAP-70 substrate linker for activation of T cells. Overexpression of Fyn, but not Lck, restored the mitogenicity of FcR nonbinding anti-CD3 in primary T cells. Taken together, these results suggest that Fyn mediates the partial signaling induced by TCR antagonists.
AB - FcR nonbinding anti-CD3ε mAbs elicit partial TCR signaling that leads to T cell unresponsiveness and tolerance in vivo. In this study, the membrane-proximal events that promote T cell inactivation by FcR nonbinding anti-CD3 mAbs were examined. In the context of FcR nonbinding anti-CD3, TCR complexes did not aggregate and failed to translocate into glycolipid-enriched membrane microdomains. Furthermore, FcR nonbinding anti-CD3 mAbs induced tyrosine phosphorylation of the Fyn substrate Cbl, but not the ZAP-70 substrate linker for activation of T cells. Overexpression of Fyn, but not Lck, restored the mitogenicity of FcR nonbinding anti-CD3 in primary T cells. Taken together, these results suggest that Fyn mediates the partial signaling induced by TCR antagonists.
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U2 - 10.4049/jimmunol.168.9.4480
DO - 10.4049/jimmunol.168.9.4480
M3 - Article
C2 - 11970992
AN - SCOPUS:0036569648
SN - 0022-1767
VL - 168
SP - 4480
EP - 4487
JO - Journal of Immunology
JF - Journal of Immunology
IS - 9
ER -