The study of human lung carcinoma cell's and mouse fibroblast's biological speciality after treated with inorganic nanometre particle

Objective: To investigate the effect of inorganic nanometre particle on the growth and reproduction of A₅₄₉ and L₉₂₉ cell lines. Methods: A₅₄₉ and L₉₂₉ cell lines were devided into control group, calcium carbonate(CaCO₃) group, hydroxyapatite( HAP) group and treated with HAP or CaCO₃ in vitro respectively, maked out the growth curve, survival rate was measured by MTT methods, and the rate of plating efficiency(PE) was measured too. Conclusions: 1 A₅₄₉'s growth cruve decreased obviously in CaCO₃ group, while there no obvious effect on L₉₂₉'s in HAP and CaCO₃ group. 2 The CaCO₃ particle's OD was 0.44 ± 0.04, 0.42 ± 0.03 at 48h, 72h respectively in L₉₂₉ cell, which were lower those of the control group (P < 0.01). 3 A₅₄₉ cell's PE in CaCO₃ group was 33. 3%, also lower than that of control group ( P < 0.01).