The use of an immunological probe to measure the kinetics of DNA repair in normal and UV-sensitive mammalian cell lines

Judith M. Clarkson; David L. Mitchell; Gerald M. Adair

doi:10.1016/0167-8817(83)90004-4

The use of an immunological probe to measure the kinetics of DNA repair in normal and UV-sensitive mammalian cell lines

Judith M. Clarkson, David L. Mitchell, Gerald M. Adair

Epigenetics & Molecular Carcinogenesis

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Abstract

Chinese hamster ovary cells and human fibroblasts were used to study UV-light-induced repair replication and removal of antibody-binding sites. Whereas repair replication still continued 8 h post irradiation, removal of antibody-binding sites was 80% complete within 2 h and reached a plateau by 4 h. This was found to be independent of the method of DNA isolation. UV-hypersensitive CHO cells exhibited reduced levels of repair synthesis that closely correlated with the extent of removal of antibody-binding sites. XP group A, C and D cells, each of which had less than 15% of the level of repair synthesis found in the control fibroblasts, removed less than 30% of the antibody-binding sites. Group E cells demonstrated intermediate levels of DNA-repair capacity in both assays.

Original language	English (US)
Pages (from-to)	287-299
Number of pages	13
Journal	Mutation Research DNA Repair Reports
Volume	112
Issue number	5
DOIs	https://doi.org/10.1016/0167-8817(83)90004-4
State	Published - Oct 1983

ASJC Scopus subject areas

General Medicine

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10.1016/0167-8817(83)90004-4

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title = "The use of an immunological probe to measure the kinetics of DNA repair in normal and UV-sensitive mammalian cell lines",

abstract = "Chinese hamster ovary cells and human fibroblasts were used to study UV-light-induced repair replication and removal of antibody-binding sites. Whereas repair replication still continued 8 h post irradiation, removal of antibody-binding sites was 80% complete within 2 h and reached a plateau by 4 h. This was found to be independent of the method of DNA isolation. UV-hypersensitive CHO cells exhibited reduced levels of repair synthesis that closely correlated with the extent of removal of antibody-binding sites. XP group A, C and D cells, each of which had less than 15% of the level of repair synthesis found in the control fibroblasts, removed less than 30% of the antibody-binding sites. Group E cells demonstrated intermediate levels of DNA-repair capacity in both assays.",

author = "Clarkson, {Judith M.} and Mitchell, {David L.} and Adair, {Gerald M.}",

note = "Funding Information: We wish to thank Judy Ing and Virginia Coy for excellent technical assistance. This work was supported by National Institute of Health grants CA 04484, CA19281, and CA 24540.",

year = "1983",

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T1 - The use of an immunological probe to measure the kinetics of DNA repair in normal and UV-sensitive mammalian cell lines

AU - Clarkson, Judith M.

AU - Mitchell, David L.

AU - Adair, Gerald M.

N1 - Funding Information: We wish to thank Judy Ing and Virginia Coy for excellent technical assistance. This work was supported by National Institute of Health grants CA 04484, CA19281, and CA 24540.

PY - 1983/10

Y1 - 1983/10

N2 - Chinese hamster ovary cells and human fibroblasts were used to study UV-light-induced repair replication and removal of antibody-binding sites. Whereas repair replication still continued 8 h post irradiation, removal of antibody-binding sites was 80% complete within 2 h and reached a plateau by 4 h. This was found to be independent of the method of DNA isolation. UV-hypersensitive CHO cells exhibited reduced levels of repair synthesis that closely correlated with the extent of removal of antibody-binding sites. XP group A, C and D cells, each of which had less than 15% of the level of repair synthesis found in the control fibroblasts, removed less than 30% of the antibody-binding sites. Group E cells demonstrated intermediate levels of DNA-repair capacity in both assays.

AB - Chinese hamster ovary cells and human fibroblasts were used to study UV-light-induced repair replication and removal of antibody-binding sites. Whereas repair replication still continued 8 h post irradiation, removal of antibody-binding sites was 80% complete within 2 h and reached a plateau by 4 h. This was found to be independent of the method of DNA isolation. UV-hypersensitive CHO cells exhibited reduced levels of repair synthesis that closely correlated with the extent of removal of antibody-binding sites. XP group A, C and D cells, each of which had less than 15% of the level of repair synthesis found in the control fibroblasts, removed less than 30% of the antibody-binding sites. Group E cells demonstrated intermediate levels of DNA-repair capacity in both assays.

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The use of an immunological probe to measure the kinetics of DNA repair in normal and UV-sensitive mammalian cell lines

Abstract

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